Effects of hormones targeting nuclear receptors on transcriptional regulation of the growth hormone gene in the MtT/S rat somatotrope cell line

Neuroendocrinology
Yasumasa IwasakiKinji Inoue

Abstract

We have examined the effects of nuclear receptor hormones such as glucocorticoid, gonadal steroid hormones, thyroid hormone and retinoids on the transcriptional regulation of the 5'-promoter activity of growth hormone (GH) gene using the MtT/S rat pure somatotrope cell line or MtT/SGL, a subclone of MtT/S in which the rat GH gene 5'-promoter (1.7 Kb)-luciferase fusion gene was stably incorporated. RT-PCR analyses revealed that receptors for all the hormones except androgen receptor were expressed in the cell line. Triiodothyronine (T(3), 10 nM) transiently but significantly stimulated the promoter activity of GH gene, whereas retinoic acids (9-cis and all-trans, both 1 microM) showed sustained stimulation. There were no additive effects among the T(3), all-trans, and9-cis retinoic acids. Synthetic glucocorticoid hormone dexamethasone (100 nM) showed an inhibitory effect but, interestingly, significantly enhanced T(3)-stimulated GH promoter activity during long-term incubation. Among the gonadal steroid hormones tested, estradiol and estriol had significant stimulatory effects, and deletion analysis showed that the estrogen effect was maintained with the shortest construct examined (-150 to +6, +1 denotes the transcription start...Continue Reading

Citations

Oct 10, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Teresinha Lermen Donatti, Vera Hermina Koch
Jul 16, 2008·Reviews in Endocrine & Metabolic Disorders·Vita BirznieceKen K Y Ho
Dec 26, 2006·Molecular and Cellular Biology·Tove I KlokkFahri Saatcioglu
Oct 24, 2008·Endocrine Journal·Chikari TakeoSatoshi Inoue
Feb 3, 2009·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Feng-ying GongYi-fan Shi
Mar 11, 2008·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Haruo Nogami, Setsuji Hisano
May 15, 2007·Nutrition·Alessia SagazioRoberto Salvatori
Sep 29, 2005·General and Comparative Endocrinology·Anitha ElangoThomas T Chen
Feb 12, 2008·Domestic Animal Endocrinology·Elpetra P M Timmermans-SprangJan A Mol
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·S ZárateA Seilicovich
Aug 9, 2008·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Feng-Ying GongYi-Fan Shi
Nov 29, 2013·Molecular Endocrinology·Dimiter AvtanskiSally Radovick

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