Effects of HPV-16 E5, E6 and E7 proteins on survival, adhesion, migration and invasion of trophoblastic cells

Carcinogenesis
Selma BoulenouarVéronique Fontaine

Abstract

Among high-risk human papillomaviruses (HPV), HPV-16 infection is the most prevalent causative factor for cervical cancer. Beside other mucosal targets, HPV-16 was reported to infect the placenta and to replicate in trophoblastic cells. Since these cells share invasive properties of tumoral cells, they represent an ideal model to investigate several oncogenic processes. In the present work, we analyzed the impacts of HPV-16 E5, E6 and E7 oncoproteins on the trophoblastic model. Our results showed that E5 impaired the viability of trophoblastic and cervical cell lines but E6 and E7, favoring cell growth, neutralized the E5 cytotoxic effect. In addition, E5 decreased the adhesiveness of trophoblastic cells to the tissue culture plastic and to endometrial cells similarly as described previously for E6 and E7. E5 and E6 plus E7 increased also their migration and their invasive properties. Cells expressing HPV-16 early proteins under the control of the long control region endogenous promoter displayed growth advantage and were also more motile and invasive compared with control cells. Interestingly, the E-cadherin was downregulated in trophoblastic cells expressing E5, E6 and E7. Nuclear factor-kappaB and activator protein-1 activit...Continue Reading

References

Feb 1, 1992·Human Pathology·M H StolerT R Broker
Aug 1, 1973·Virology·F L Graham, A J van der Eb
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A S YapB M Gumbiner
Dec 26, 2001·Oncogene·D DiMaio, D Mattoon
Nov 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Jean-Louis JanneauDominique Bellet
Jul 2, 2003·Virology·Gary L DisbrowRichard Schlegel
Oct 27, 2004·Trends in Biochemical Sciences·Neelan J MarianayagamJacqueline M Matthews
Nov 16, 2005·Reviews in Medical Virology·Christy M Hebner, Laimonis A Laimins
Feb 1, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M IshikawaS Nozawa
Jun 2, 2007·Annual Review of Cell and Developmental Biology·Sabine Pokutta, William I Weis
Aug 9, 2008·The American Journal of Pathology·Ewa KrawczykRichard Schlegel
Aug 19, 2008·Biochemical Pharmacology·Paola Chiarugi, Elisa Giannoni
Sep 11, 2008·Current Pharmaceutical Design·Ala-Eddin Al MoustafaAmber Yasmeen
Nov 5, 2008·Cancer·Martin A WhitesideElizabeth R Unger

❮ Previous
Next ❯

Citations

Jan 7, 2011·The Journal of Infectious Diseases·Christine WeynVéronique Fontaine
Apr 20, 2013·The Journal of Infectious Diseases·Paul A OrlandoAnna R Guliano
Sep 20, 2012·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Mireille MerckxDavy Vanden Broeck
Oct 22, 2013·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shu-Jie LiaoDing Ma
Feb 9, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A C FreitasA L S Jesus
Jan 12, 2013·Archives of Gynecology and Obstetrics·Linda J HongPhilip J Chan
Apr 26, 2016·Infectious Diseases in Obstetrics and Gynecology·Lea Maria Margareta AmbühlSuzette Sørensen
Sep 9, 2015·International Journal of Reproductive Medicine·Jennifer A TolenPhilip J Chan
Oct 8, 2013·American Journal of Obstetrics and Gynecology·Mollie McDonnoldMaged M Costantine
Sep 18, 2014·Archives of Gynecology and Obstetrics·Sarah S ChenPhilip J Chan
Jul 2, 2016·Virusdisease·Damiano PizzolKajal D Chhaganlal
Jan 11, 2017·British Journal of Cancer·Marine LefevreUNKNOWN Papillophar Study Group
Sep 15, 2017·Pathogens and Disease·Moises León-JuárezEnrique Reyes-Muñoz
Jun 18, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Stina Syrjänen
Jun 10, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xue ChenYa Cao
Jun 5, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ming-Xin CaoYa-Ling Tang
Mar 21, 2019·Journal of Cellular Physiology·Javid Sadri NahandHamed Mirzaei
Feb 3, 2018·Molecular Medicine Reports·Jianhui ZhangShun Zhang
Mar 27, 2020·Pathogens·Ashley L Reily-BellTania L Slatter
Oct 28, 2020·Molecular Cancer Research : MCR·Lourdes Gutierrez-XicotencatlFernando Esquivel-Guadarrama
Jul 14, 2021·Reproductive Sciences·Meiyuan JinChao Tang
Sep 24, 2021·Biotechnology and Applied Biochemistry·F Nadhirah JaaparIffah Izzati Zakaria
Dec 29, 2021·Congenital Anomalies·Zahra HeydarifardNazanin-Zahra Shafiei-Jandaghi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.