Effects of human diabetic serum on the in vitro development of mouse preimplantation embryos

Teratology
I ZusmanA Ornoy

Abstract

The effects of sera from different types of human diabetes (type I with and without ketoacidosis; type II treated with insulin or Daonil or untreated) on the in vitro development of early preimplantation mouse embryos were studied. In controls, 20% of blastocysts failed to develop successfully when grown for 72 h in RPMI medium supplemented with 10% fetal bovine serum and 50% nondiabetic human serum. In experiments using 50% diabetic serum, the highest embryotoxic effect was found in type-I diabetes with and without ketoacidosis: The percents of undeveloped embryos were 66 and 58, respectively. In type-II diabetes, embryotoxic effects were found among all studied types: The percent of undeveloped blastocysts varied from 36% in insulin-treated type-II diabetes to 44% in untreated type-II diabetes. A high correlation was found between the number of undeveloped embryos and the blood concentrations of metabolic diabetic factors: glucose (r = .53-.64 in type-I diabetes), B-HOB (r = .7-.77 in type-II diabetes untreated or treated with Daonil), acetoacetate (r = .66 in insulin-treated type-II diabetes), and HbA1c (r = .89 in insulin-treated type-II diabetes or .99 in Daonil-treated type-II diabetes). A concentration of 80% serum was e...Continue Reading

References

Jan 1, 1976·Annual Review of Physiology·J D Biggers, R M Borland
May 1, 1987·American Journal of Obstetrics and Gynecology·T C KeyT R Moore
May 1, 1987·American Journal of Obstetrics and Gynecology·H Kalter
Jul 1, 1987·American Journal of Obstetrics and Gynecology·T W Sadler, E S Hunter
Oct 15, 1985·American Journal of Obstetrics and Gynecology·M MiodovnikT A Siddiqi
Oct 15, 1984·American Journal of Obstetrics and Gynecology·M MiodovnikS J Stys
Aug 1, 1980·In Vitro·A Spindle

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Citations

Oct 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A OrnoyI Raz
Feb 1, 1990·Biological Reviews of the Cambridge Philosophical Society·I Zusman, A Ornoy
Jun 25, 2009·The Journal of Reproduction and Development·Xing-Hui ShenNam-Hyung Kim

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