PMID: 30003752Jul 14, 2018Paper

Effects of human enhancer of filamentation 1 (HEF1) gene on the proliferation, invasion and metastasis of bladder cancer cells

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Zhiling Tang

Abstract

To investigate the effects of human enhancer of filamentation 1 (HEF1) gene on the proliferation, invasion and metastasis of bladder cancer cells. Three human bladder cancer cell lines (T24, EJ and BIU-87) were selected to extract total RNA at logarithmic growth phase. The relative expression level of HEF1 in these cell lines was detected by semi-quantitative reverse transcription PCR (RT-PCR), and the cell lines with relatively high expression and relatively low expression level of HEF1 cells were identified. HEF1 overexpression recombinant adenovirus was transfected into the bladder cancer cells with low expression level of HEF1, and HEF1 siRNA was transfected into the bladder cancer cells with high expression level of HEF1. MTT assay, migration assay and invasion assay were performed to detect the proliferation, migration and invasion of bladder cancer cells. The relative expression level of HEF1 mRNA in T24 cell line was significantly higher than that in EJ and BIU-87 cells, and BIU-87 cell line showed the lowest expression level (p<0.05). After transfection with HEF1 overexpression recombinant adenovirus, the proliferation, migration and invasion of BIU-87 cells were significantly improved (p<0.05). After siRNA silencing, ...Continue Reading

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