Effects of human lactoferrin on the cytoplasmic membrane of Candida albicans cells related with its candidacidal activity

FEMS Immunology and Medical Microbiology
Mónica Viejo-DíazJosé F Fierro

Abstract

Human lactoferrin is an innate host defence protein with antimicrobial activity that exerts a candidacidal effect in a cation concentration-dependent manner. We investigated the ability of this cationic protein (with an isoelectric point of 8.7) to permeabilize the cytoplasmic membrane of Candida albicans cells. Despite minor K(+)-release in lactoferrin-treated C. albicans cells, the killing effect was not related to an extensive membrane permeabilization, as indicated by: (a) the non-release of macromolecular cytosolic constituents; (b) the non-permeabilization for extracellular propidium iodide nor for intracellular accumulated calcein; and (c) the inability to disrupt the phospholipid bilayer of 8-aminonaphthalene-1,3,6, trisulfonic acid/p-xylene-bis-pyridiniumbromide-loaded liposomes. These results suggest that lactoferrin exerts its candidacidal effect through a mechanism different from membrane permeabilization described for other cationic peptides.

References

May 22, 1992·Biochimica Et Biophysica Acta·W BellamyM Tomita
Apr 1, 1974·Journal of General Microbiology·S M HammondB N Kliger
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W R Jarvis
May 1, 1995·Journal of Dental Research·R D CannonB C Monk
Dec 1, 1993·Archives of Oral Biology·H NikawaT Hamada
Oct 1, 1996·Clinical Microbiology Reviews·S K Fridkin, W R Jarvis
Feb 1, 1997·The Journal of Antimicrobial Chemotherapy·D ZakulaR C Goldman
Dec 31, 1998·Antimicrobial Agents and Chemotherapy·A J De Lucca, T J Walsh
Jan 16, 1999·Biochemical Pharmacology·J M Schröder
Mar 6, 1999·The Journal of Biological Chemistry·E J HelmerhorstT Abee
Apr 14, 1999·Medical Mycology·Y Y XuH Nikawa
May 29, 1999·Antimicrobial Agents and Chemotherapy·R E Hancock, D S Chapple
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·A LupettiP H Nibbering
Jan 4, 2001·Journal of Clinical Microbiology·F PeyronA Bolmström
Mar 10, 2001·Biochemistry. Biokhimii︠a︡·T G KanyshkovaG A Nevinsky
Mar 1, 2002·Journal of Microbiological Methods·Dmitri S OrlovRobert I Lehrer
Oct 22, 2003·Molecular Immunology·Deirdre A Devine
Feb 25, 2005·FEMS Microbiology Letters·Hisako YamagishiBrian C O'Connell

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Citations

Aug 20, 2008·Antimicrobial Agents and Chemotherapy·María T AndrésJosé F Fierro
Feb 6, 2013·Cellular and Molecular Life Sciences : CMLS·Nicole L van der WeerdenMarilyn A Anderson
Sep 23, 2011·Biochemical Society Transactions·Katrijn De BruckerKarin Thevissen
Mar 15, 2008·Médecine et maladies infectieuses·J LabidiA Bouzaiene
May 4, 2005·Yeast
Nov 24, 2019·International Journal of Molecular Sciences·María T AndrésJosé F Fierro
Oct 8, 2019·International Journal of Medical Microbiology : IJMM·Julio C CarreroMireya de la Garza

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