Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae.

Infection and Immunity
P Buck, R F Rest

Abstract

Neisseria gonorrhoeae were exponentially killed for 120 min (i.e., they were prevented from forming colonies on agar) by extracts of human neutrophil granules; however, macromolecular synthesis, indicated by incorporation of radiolabeled precursors in trichloroacetic acid-precipitable material, continued at or above zero time control values for 45 min. Protein, deoxyribonucleic acid, and ribonucleic acid synthesis appeared to decrease simultaneously after 45 min. Little or no lysis gonococci occurred during the first 60 min of incubation. The ions K+, Na+, Ca2+, Cl1-, SO4(2-) and PO4(3-) at concentrations of less than or equal to 100 mM did not affect granule extract bactericidal activity. On the other hand, 20 mM Mg2+ completely inhibited killing when initially present along with granule extract or when added within 2 to 5 min after granule extract was added to a suspension of gonococci. Gonococci treated with granule extract, washed, and then incubated in gonococci. Gonococci treated with granule extract, washed, and then incubated in the absence of extract died as if extract were still present. The ability of subinhibitory concentrations of actinomycin D or erythromycin to inhibit growth and protein and nucleic acid synthesi...Continue Reading

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Citations

Aug 17, 2011·Infection and Immunity·Adrienne Chen, H Steven Seifert
Aug 1, 1990·Molecular Microbiology·W M ShaferL W Huthwaite
Apr 1, 1983·Infection and Immunity·R L Hodinka, M C Modrzakowski
Oct 1, 1986·Infection and Immunity·W M ShaferL E Martin
Apr 1, 1989·Clinical Microbiology Reviews·R F Rest, W M Shafer

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