Effects of hyaluronic acid in culture and cytochalasin B treatment before freezing on survival of cryopreserved bovine embryos produced in vitro.

In Vitro Cellular & Developmental Biology. Animal
M Franco, P J Hansen

Abstract

One limitation to the widespread use of in vitro-produced embryos in cattle is their poor survival following cryopreservation. Two approaches for enhancing survival of in vitro-produced bovine embryos following cryopreservation were evaluated: culture in the presence of hyaluronic acid and alterations in the cytoskeleton through cytochalasin B treatment. The experiment was a 2 x 2 factorial design to test main effects of hyaluronic acid added to culture at day 5 after insemination (+ or -) and cryopreservation treatment (control or cytochalasin B). Embryos used for cryopreservation were blastocysts and expanded blastocysts harvested on day 7 after insemination. Cytochalasin B increased the percent of embryos that re-expanded (P < 0.0001) and that hatched following thawing (P < 0.05). The hatching percent was 29.6% for embryos treated with cytochalasin B versus 9.1% for control embryos. There was no significant effect of hyaluronic acid on survival although there was a tendency for embryos cultured with hyaluronic acid to have higher percent hatching if not treated with cytochalasin B (12.7% for hyaluronic acid versus 4.5% for control; hyaluronic acid x cytochalasin B interaction; P = 0.09). In conclusion, cytochalasin B treatme...Continue Reading

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Citations

Jul 23, 2015·In Vitro Cellular & Developmental Biology. Animal·L M G PaimA T D Oliveira
Oct 31, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Nobuyuki Matubayasi
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Alejandro CuetosLuis F Rull
Oct 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Matthias Schmidt
Sep 11, 2010·Neonatal Network : NN·Andrea L Randenberg
Nov 9, 2010·Cryobiology·Vishard RagoonananAlptekin Aksan

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