PMID: 16520532Mar 8, 2006Paper

Effects of hyaluronic acid on Ca(2+) influx, lactate dehydrogenase activity, and cyclic AMP synthesis in canine ejaculated sperm during In Vitro capacitation

The Journal of Veterinary Medical Science
Eiichi KawakamiT Tsutsui

Abstract

The effects of hyaluronic acid, which comprises the cumulus intercellular matrix, on Ca(2+) influx, lactate dehydrogenase (LDH) activity, and cyclic AMP synthesis in canine sperm during capacitation was investigated. Ejaculated sperm were collected from 10 Beagle dogs and the sperm were incubated for 4 hr in Eagle's MEM containing 10 microg/ml of hyaluronic acid. The percentages of actively motile sperm, hyperactivated sperm (HA-sperm), acrosome-reacted sperm (AR-sperm), and sperm labeled with fluoresceinated Ca(2+) indicator (Ca(2+)-labeled sperm) were evaluated to assess Ca(2+) influx into the sperm. LDH activity and cAMP concentration were measured in homogenized sperm. The mean percentages of motile sperm, HA-sperm, and Ca(2+)-labeled sperm in the MEM containing hyaluronic acid were higher than in the control medium (P<0.05, 0.05, and 0.01, respectively), but there was no difference between the percentages of AR-sperm. Mean LDH activity and mean cAMP concentration were also significantly higher than the control values (P<0.05). The percentages of HA-sperm correlated with those of Ca(2+)-labeled sperm (r(2)=0.810). The results indicate that hyaluronic acid increases Ca(2+) influx, LDH activity, and cAMP synthesis in canine e...Continue Reading

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Jun 10, 2004·The Journal of Veterinary Medical Science·Eiichi KawakamiToshihiko Tsutsui

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Citations

Apr 28, 2009·Biological Trace Element Research·Na LiFashui Hong
Nov 2, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Ali A Fouladi-NashtaGeraldine M Hartshorne
Sep 25, 2014·Reproduction in Domestic Animals = Zuchthygiene·S Fernández, M Córdoba
Nov 24, 2020·Andrologia·Ivan Cunha Bustamante-FilhoMaria Inês Mascarenhas Jobim

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