PMID: 6165781Jun 1, 1981Paper

Effects of hyaluronidase and neuraminidase on immunoreactivity histidine-rich protein in newborn rat epidermis

The Journal of Investigative Dermatology
H KimuraW L Epstein

Abstract

Frozen sections of newborn rat skin were treated with a variety of buffers, enzymes, and proteinase inhibitors in order to modify the reactivity of antigenic sites of histidine-rich protein and keratin. By indirect immunofluorescence, we found that antiserum to histidine-rich protein purified from granular cells reacted with keratohyalin granules but not cornified cells treated with hyaluronidase. The same antiserum reacted less distinctly with keratohyalin granules treated with neuraminidase; in contrast, it showed strong reactivity in cornified cells after the neuraminidase digestion. However, the enzyme digestions did not unmask the antigenic site(s) of keratohyalin granules to anti-keratin serum, as did saline in 0.1 M Tris-HCl buffer, pH 8.0. These results suggest that the antigenic site of histidine-rich protein is masked in keratohyalin granules by different mechanisms from the masking of keratin. Histindine-rich protein may be masked primarily with hyaluronic acid in keratohyalin granules, but the sugar moiety appears to be changed to sialic acid in cornified cells.

Citations

Dec 1, 1994·The Journal of Dermatologic Surgery and Oncology·L E Clark, J R Mellette
Jul 1, 1990·The Journal of Investigative Dermatology·A ReanoJ Thivolet

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