PMID: 3768374Nov 7, 1986Paper

Effects of hydration in the oxygenation and autoxidation of carbonmonoxyhemoglobin powder

Biochimica Et Biophysica Acta
R SanchesM F Colombo

Abstract

The oxygenation and autoxidation of lyophilized human carbonmonoxyhemoglobin (Hb-CO) kept in environments with different relative humidities were followed with time using visible absorption spectroscopy. The sample kept at 68% relative humidity was the one with the greatest formation of methemoglobin (Met-Hb), while for increasing or decreasing hydrations the Met-Hb content decreased gradually. Besides the presence of Met-Hb, it was observed that the samples kept above about 68% relative humidity were converted to oxyhemoglobin (Hb-O2), the samples kept in the range 45-68% relative humidity were a mixture of Hb-CO and Hb-O2, while the samples kept below about 45% relative humidity and the solution sample continued as Hb-CO. Hemoglobin has, therefore, two critical hydration values, 45% and 68% relative humidity, which correspond to about 0.12 and 0.18 g H2O/g protein, respectively.

References

Aug 15, 1979·Journal of Molecular Biology·D A Case, M Karplus
Sep 1, 1976·British Medical Bulletin·M F Perutz
May 1, 1977·FEBS Letters·S Böhm, L V Abaturov
Sep 1, 1968·The Journal of Physical Chemistry·G BrausseH Vogel
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daniel M Czajkowsky, Zhifeng Shao
Dec 1, 1972·The Journal of Membrane Biology·M J Schneider, A S Schneider

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Citations

Nov 1, 1990·Plant Physiology·C W Vertucci, E E Roos
Feb 21, 2002·Pharmaceutical Development and Technology·Kenneth C WatermanChristopher J Roberts
Aug 10, 1988·Biochimica Et Biophysica Acta·R Sanches
Mar 1, 1989·Journal of Pharmaceutical Sciences·P LabrudeB Chaillot

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