Effects of hydrogen peroxide and hydroxyl radicals on the cytosolic side of a non-selective cation channel in the cultured human bronchial epithelial cell line 16HBE14o-

Pflügers Archiv : European journal of physiology
C JeulinF Marano

Abstract

Respiratory tissues can be damaged by the exposure of airway epithelial cells to reactive oxygen species (ROS) that generate an oxidative stress. We studied the effects of the hydroxyl radical *OH, for which there is no natural intra- or extracellular scavenger, on a Ca2+-activated, non-selective cation channel (NSC(Ca)) which might participate in the transepithelial Na+ fluxes involved in the maintenance of the cytosolic [Na+] ([Na+]i). We identified and characterized NSC(Ca) in inside-out excised membrane patches from cells of the human bronchial cell line 16HBE14o- and exposed the cytoplasmic side of NSC(Ca) to H2O2 or *OH created in front of the patch pipette. In these cells, the NSC channel was Ca2+ (above 0.1 microM) and voltage dependent. The channel's low open probability ( P(o)) at hyperpolarized voltages increased rapidly to a very high value upon short exposure (seconds) to *OH or to H2O2 in a bath solution containing millimolar [Ca2+]. This sensibility to ROS was reversible on wash-out of the oxidants. Long exposure (several minutes) to *OH in a bath solution containing 10(-7) M Ca2+ activated the channel irreversibly, a finding that has pathophysiological implications. The functioning of the NSC(Ca) protein channel...Continue Reading

Citations

May 11, 2006·The Journal of Membrane Biology·R FrancoJ A Cidlowski
Oct 16, 2012·Pflügers Archiv : European journal of physiology·J Marc SimardVolodymyr Gerzanich
Apr 6, 2004·Brain Research·Deok-Jin ChangBong-Kiun Kaang
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anne-Catherine DazyFrancelyne Marano
Oct 5, 2010·The Journal of Biological Chemistry·Felipe SimonAndrés Stutzin
May 28, 2004·American Journal of Physiology. Cell Physiology·Felipe SimonAndrés Stutzin
Sep 9, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette JeulinFrancelyne Marano
Jun 5, 2003·Journal of Neurophysiology·Fivos Vogalis, John R Harvey
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette JeulinFrancelyne Marano
Nov 19, 2004·Journal of Muscle Research and Cell Motility·Kiril L HristovDessislava B Duridanova

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