PMID: 22333057Feb 16, 2012Paper

Effects of hydrogen sulfide on ouabain-induced delayed after-depolarization and triggered activity in guinea pig papillary muscles

Zhonghua yi xue za zhi
Hui-xia LiuYu-ming Wu

Abstract

To explore the effects of hydrogen sulfide (H(2)S) on delayed after-depolarization (DAD) and triggered activity induced by ouabain in male guinea pig papillary muscles and to elucidate the underlying mechanisms. An intracellular microelectrode was used to record the patterns of DAD and triggered activity by K-H solution containing ouabain and a high concentration of calcium ion. The latent period, amplitude, duration of DAD and incidence of triggered activity were observed under a pre-treatment with different concentrations of NaHS (donor of H(2)S). The effects of glibenclamide, Bay K8644 and NG-nitro-L-arginine methyl ester (L-NAME) pretreatment on the actions of H(2)S were also studied. NaHS (100, 200 µmol/L) prolonged the latent period of DAD from (12.0 ± 1.0) min to (19.9 ± 1.6) min (P < 0.05), (23.7 ± 1.3) min (P < 0.01), decreased the altitude of DAD from (11.47 ± 0.74) mV to (6.47 ± 0.33) mV, (5.65 ± 0.26) mV (both P < 0.01), shortened the duration of DAD from (205 ± 11) ms to (173 ± 10) ms and (134 ± 7) ms (both P < 0.05). The occurrence of triggered activity was inhibited from 5 samples to 4, 2 and 1 sample in 6 samples. A pretreatment of adenosine triphosphate (ATP)-sensitive potassium channel (K(ATP)) blocker glibenc...Continue Reading

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