PMID: 22333058Feb 16, 2012Paper

Effects of hydrogen sulfide on the expressions of nuclear factor-κB and intercellular adhesion molecule 1 in pulmonary tissue of rats with acute lung injury

Zhonghua yi xue za zhi
Meng LiJunbao Du

Abstract

To explore the potential effects of hydrogen sulfide (H(2)S) on nuclear factor kappa B (NF-κB) and intercellular adhesion molecule 1 (ICAM-1) in lung tissue with oleic acid (OA)-induced acute lung injury (ALI) in rats. Forty-two rats were randomly divided into 3 groups: control (n = 6), OA (n = 18) and OA + NaHS (n = 18). Rats in the OA group received an intra-tail vein injection of oleic acid 0.1 ml/kg while those in the OA + NaHS group an intraperitoneal injection of NaHS 56 µmol/kg at 30 mins before OA injection. The OA and OA + NaHS groups were subdivided into 3 subgroups depending on the therapeutic duration: 2 h (n = 6), 4 h (n = 6) and 6 h (n = 6). Rats in the control group received an intra-tail vein injection of normal saline 0.1 ml/kg. Bronchioalveolar lavage fluid (BALF) was collected and the leucocytic differential count of sediment examined. The extent of lung injury was evaluated by the index of quantitative assessment (IQA). The H(2)S level in lung tissue was measured by sensitive sulphur electrode. The nuclear translocation of NF-κB and the expression of ICAM-1 in alveolar epithelial cells were measured by immunohistochemical staining. Compared with the control group, the BALF percentage of polymorphonuclear (PM...Continue Reading

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