Effects of Hydrophobic Amino Acid Substitutions on Antimicrobial Peptide Behavior

Probiotics and Antimicrobial Proteins
Kimberly D Saint JeanGregory A Caputo

Abstract

Antimicrobial peptides (AMPs) are naturally occurring components of the immune system that act against bacteria in a variety of organisms throughout the evolutionary hierarchy. There have been many studies focused on the activity of AMPs using biophysical and microbiological techniques; however, a clear and predictive mechanism toward determining if a peptide will exhibit antimicrobial activity is still elusive, in addition to the fact that the mechanism of action of AMPs has been shown to vary between peptides, targets, and experimental conditions. Nonetheless, the majority of AMPs contain hydrophobic amino acids to facilitate partitioning into bacterial membranes and a net cationic charge to promote selective binding to the anionic surfaces of bacteria over the zwitterionic host cell surfaces. This study explores the role of hydrophobic amino acids using the peptide C18G as a model system. These changes were evaluated for the effects on antimicrobial activity, peptide-lipid interactions using Trp fluorescence spectroscopy, peptide secondary structure formation, and bacterial membrane permeabilization. The results show that while secondary structure formation was not significantly impacted by the substitutions, antibacterial a...Continue Reading

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Citations

Feb 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eric M KohnGregory A Caputo
Sep 29, 2018·International Journal of Molecular Sciences·Changbao SunJuncai Hou
Sep 6, 2020·Probiotics and Antimicrobial Proteins·Morgan A HitchnerGregory A Caputo
Jul 25, 2019·Current Medicinal Chemistry·Bruno CasciaroMaria Luisa Mangoni
Aug 14, 2020·Canadian Journal of Microbiology·Shikha Kapil, Vipasha Sharma
Nov 15, 2020·Probiotics and Antimicrobial Proteins·Jie PeiXian Guo
Mar 30, 2021·Computational and Structural Biotechnology Journal·Carolina Gutiérrez-ChávezBelinda C Ferrari
May 4, 2021·Peptide Science·Alexandria S SenetraGregory A Caputo
Aug 18, 2020·Protein and Peptide Letters·Matthew R NecelisGregory A Caputo
Sep 11, 2020·ACS Applied Materials & Interfaces·Haoning GongJian Ren Lu
May 11, 2019·Biochimica Et Biophysica Acta. Biomembranes·Morgan A HitchnerGregory A Caputo

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