PMID: 2501277May 1, 1989Paper

Effects of hypercapnia on brain pHi and phosphate metabolite regulation by 31P-NMR

Journal of Applied Physiology
M NishimuraH Kazemi

Abstract

The ability of brain cells to regulate intracellular pH (pHi) and several phosphate metabolites was evaluated during 1 h of hypercapnia (inspiratory CO2 fraction of 0.10 and 0.05) in anesthetized rats by 31P high-field (145.6 MHz) nuclear magnetic resonance spectroscopy. Body temperature was maintained at 37 +/- 0.5 degrees C. Fully relaxed spectra were obtained for controls and 30-50 min after CO2 loading and CO2 withdrawal. Spectra were taken serially every 2.5 min after gas mixtures were changed. Brain pHi decreased 0.10 +/- 0.02 units [7.06 +/- 0.01 (SE)] to 6.96 +/- 0.01 (P less than 0.001) after 30-50 min of 10% CO2 breathing, and arterial pH decreased 0.24 +/- 0.01 units. Brain pHi decreased by 0.045 +/- 0.01 units (7.05 +/- 0.01 to 7.01 +/- 0.01, P less than 0.05) during 5% CO2 breathing. Brain pHi returned to control values after 30-50 min of CO2 washout in both groups. In three of six animals breathing 10% CO2, there was an undershoot in brain pHi by 0.07-0.09 units between 2.5 and 20 min of hypercapnia. Three animals exhibited an overshoot in pHi by 0.06-0.11 units between 7.5 and 17.5 min during CO2 washout. Phosphocreatine-to-Pi and Pi-to-beta-ATP ratios changed during hypercapnia and returned to base line after wi...Continue Reading

Citations

Sep 1, 1992·Acta Physiologica Scandinavica·C H ThompsonG K Radda
Apr 21, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Varsha JainFelix W Wehrli
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Mykyta M ChernovJ C Leiter
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S NottinghamJ S Erlichman
Jul 23, 2013·The Journal of Experimental Biology·Li ZhangChris M Wood

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