PMID: 8967453May 1, 1996Paper

Effects of hypercapnia on prostanoid and cAMP production by cerebral microvascular cell cultures

The American Journal of Physiology
H Parfenova, C W Leffler

Abstract

In the newborn pig cerebral circulation, arteriolar dilation in response to hypercapnia requires the presence of intact endothelium and is accompanied by an indomethacin-sensitive increase in cortical adenosine 3',5'-cyclic monophosphate (cAMP). The effects of short-term hypercapnia on production of dilator prostanoids and cAMP were investigated using newborn pig cerebral microvascular smooth muscle cells and endothelial cells cultured both separately and in noncontact coculture. Microvascular smooth muscle cells respond to hypercapnia (pH 7.00 +/- 0.05 PCO2 75 +/- 3 mmHg) by a 1.3- to 1.7-fold increase in basal cAMP production that is not affected by indomethacin, whereas hypercapnia and 80 mM sodium propionate do not affect iloprost-stimulated cAMP production. Microvascular endothelial cells cultured on Millicel inserts respond to hypercapnia by a two- to fourfold increase in prostacyclin (as 6-keto-prostaglandin F1 alpha) and prostaglandin E2 production in both luminal and abluminal compartments. For noncontact coculture, Millicel inserts with endothelial cells (as hypercapnia-sensitive producers of prostanoids) were installed into cell culture dishes with aspirin-pretreated smooth muscle cells (as targets for endothelium-de...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C Iadecola
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P GanzR W Alexander
Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D A PelligrinoR F Albrecht

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Citations

Jan 13, 2000·Proceedings of the Society for Experimental Biology and Medicine·P A ParkinsonC W Leffler
Aug 7, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K KomjátiP Sándor
Oct 13, 2010·European Journal of Applied Physiology·Jui-Lin FanPhilip N Ainslie
Sep 6, 2003·American Journal of Respiratory and Critical Care Medicine·John G LaffeyPaul McLoughlin
Oct 18, 2007·The Korean Journal of Internal Medicine·Jin Hwa LeeJung Hyun Chang
Jan 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jan WenzelMarkus Schwaninger
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·K ShibataB P Kavanagh
Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H ParfenovaC W Leffler
Oct 11, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Alie Kanu, Charles W Leffler
Oct 12, 1999·The American Journal of Physiology·H ParfenovaC W Leffler
Sep 1, 2004·Experimental Biology and Medicine·Massroor PourcyrousCharles W Leffler
Aug 10, 2000·American Journal of Respiratory and Critical Care Medicine·J G LaffeyB P Kavanagh
Dec 5, 2021·Pediatric Research·Sandeep K ChilakalaMassroor Pourcyrous

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