PMID: 9543496May 16, 1998Paper

Effects of hyperosmolality on Na, K-ATPase gene expression in vascular smooth muscle cells

The Journal of Membrane Biology
S MutoY Asano

Abstract

Cultured vascular smooth muscle cells (VSMC) from rat thoracic aortas were exposed to hyperosmotic media to determine the effects on Na, K-ATPase alpha1- and beta1-mRNA expression. Hyperosmotic media (500 mOsm/kgH2O) supplemented with glucose or mannitol increased alpha1-mRNA levels threefold at 24 hr and beta1-mRNA levels sevenfold at 12 hr. In sharp contrast, hyperosmotic urea medium had no effect at any time. Both the protein synthesis inhibitor cycloheximide and the RNA transcription inhibitor actinomycin D reduced alpha1- and beta1-mRNA upregulation induced by hyperosmotic glucose or mannitol media. Protein kinase C (PKC) inhibitors (staurosporine A or calphostin C) or tyrosine kinase (TK) inhibitors (genistein or herbimycin A) had no effect on the alpha1-mRNA upregulation induced by hyperosmotic glucose or mannitol media. Hyperosmotic glucose or mannitol media (500 mOsm/kgH2O) significantly increased alpha1- and beta1-subunit protein levels and Na, K-ATPase activity, whereas hyperosmotic urea medium had no effect. Transfection experiments with the 5'-flanking sequences of the alpha1- or beta1-subunit genes linked to the luciferase reporter gene revealed that hyperosmolar glucose medium increased luciferase activity 2.9- a...Continue Reading

Citations

Nov 19, 2015·Frontiers in Cell and Developmental Biology·Zhiqin Li, Sigrid A Langhans
Jun 6, 2000·American Journal of Physiology. Cell Physiology·Y MiyataY Asano
May 15, 2001·American Journal of Physiology. Cell Physiology·N SevieuxE Songu-Mize
Apr 9, 2001·American Journal of Physiology. Renal Physiology·J M CapassoT Berl
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Devulapalli ChakravartyDietmar Kültz
Jul 29, 2004·The Journal of Biological Chemistry·Randall K WetzelElena Arystarkhova
May 18, 1999·Biochemical and Biophysical Research Communications·M HayashiT Fujita
Jun 13, 2012·Journal of Proteome Research·Hyo-Jung ChoiTae-Hwan Kwon

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