Effects of hyperosmotic solutions on the filament lattice of intact frog skeletal muscle

Biophysical Journal
B M MillmanI Matsubara

Abstract

The effect of increasing the osmotic strength of the extracellular solution on the fifament lattice of living frog sartorius and semitendinosus muscle has been studied using low-angle x-ray diffraction to measure the lattice spacing. As the extracellular osmotic strength is increased, the filament lattice shrinks like an osmometer until a minimal spacing between the thick filaments is reached. This minimal spacing varies from 20 to 31 nm, depending on the sarcomere length. Further increase in the osmotic strength produces little further shrinkage. The osmotic shrinkage curve indicates, for both muscles, an osmotically-inactive volume of approximately 30% of the volume in normal Ringer's solution. Shrinkage appears to be independent of temperature and the type of particle used to increase the osmotic strength (glucose, sucrose, small ions). The rate at which osmotic equilibruim is reached depends on muscle size, being slower for greater muscle diameters. Equilibrium spacings are approached exponentially with time constants ranging from 20 to 60 min. Independent of osmotic equilibrium, the lattice tends to shrink slowly by approximately 3% over the first few hours after dissection, probably because of a leakage of K+ ions from in...Continue Reading

References

Dec 30, 1972·Journal of Molecular Biology·I Matsubara, G F Elliott
Apr 14, 1967·Journal of Molecular Biology·G F ElliottB M Millman
Oct 1, 1980·Biophysical Journal·B M Millman, B G Nickel

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Citations

Feb 1, 1996·International Journal of Biological Macromolecules·C R Worthington, G F Elliott
Sep 1, 2005·Meat Science·Elisabeth Huff-Lonergan, Steven M Lonergan
Dec 1, 1996·International Journal of Biological Macromolecules·C R Worthington, G F Elliott
Jan 1, 1985·Critical Reviews in Food Science and Nutrition·A AsgharT Yasui
May 17, 2005·Biophysical Journal·Masako KimuraYoshiki Umazume
Sep 23, 2016·Biophysical Journal·Toshiki ShimomuraMadoka Suzuki
Feb 1, 1992·Journal of Muscle Research and Cell Motility·T C Irving, B M Millman
Apr 30, 1998·Physiological Reviews·B M Millman
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Gaëlle PetitJean-Luc Martin

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