PMID: 11341946May 9, 2001Paper

Effects of hyperoxia and iron on iron regulatory protein-1 activity and the ferritin synthesis in mouse peritoneal macrophages

Biochimica Et Biophysica Acta
K Kuriyama-MatsumuraS Bannai

Abstract

Ferritin is an intracellular iron storage protein and its translation is inhibited by binding of iron regulatory proteins (IRPs) to the iron-responsive element (IRE) located in the 5' untranslated region of its mRNA. In this paper, we have investigated the effect of hyperoxia and iron on the binding activity of IRP-1 and the ferritin synthesis in mouse peritoneal macrophages. The binding activity of IRP-1 was increased and the ferritin synthesis was suppressed when the macrophages were cultured under hyperoxia, and the reverse occurred under hypoxia. Iron diminished the IRP-1-binding activity and the enhanced synthesis of ferritin. However, this effect was arrested under hyperoxia. Consistently, hypoxia-induced loss of binding activity of IRP-1 and the enhanced synthesis of ferritin were blocked in the presence of an iron chelator deferoxamine. These alterations of the binding activity of IRP-1 in response to oxygen and iron were not reproduced in the cell-free extract. The data suggest that in the macrophages oxygen and iron inversely act on the binding activity of IRP-1 and the ferritin synthesis, and that intracellular mechanism(s) to sense iron and/or oxygen is required for these actions.

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Citations

Apr 6, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna Cook Abt, Olimpia Meucci
May 23, 2003·The Journal of Laboratory and Clinical Medicine·Des R Richardson
Dec 28, 2010·Free Radical Biology & Medicine·Nikolai L Chepelev, William G Willmore
May 1, 2002·Redox Report : Communications in Free Radical Research·Carine Fillebeen, Kostas Pantopoulos
Apr 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexandra K KiemerAngelika M Vollmar
Mar 12, 2003·Journal of Leukocyte Biology·Thomas F MuellerDavid L Perkins
Jul 12, 2003·Blood·Brian D Schneider, Elizabeth A Leibold
May 3, 2002·Blood·Frank M Torti, Suzy V Torti
Jan 26, 2021·Drug Discovery Today·Prakash VasanthiDharmalingamRajarajan A Thandavarayan
Mar 11, 2004·The Journal of Experimental Biology·Kevin Larade, Kenneth B Storey
Jul 3, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Prihartini WidiyantiSoetjipto Soetjipto

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