Effects of hypoxia and low temperature on substrate fluxes in fish: plasma metabolite concentrations are misleading

The American Journal of Physiology
F HamanJ M Weber

Abstract

Oxygen levels and temperature can fluctuate rapidly in aquatic environments. Ever though the effects of environmental stresses on fish metabolism have been studied extensively, information on fue kinetics is extremely limited because it relies almost exclusively on changes in substrate concentrations. The turnover rate of nonesterified fatty acids (NEFA) has never been measured in fish. Therefore, our goal was to quantify glucose and NEFA fluxes in rainbow trout acutely exposed to severe hypoxia (25% O2 saturation) or low temperature (6 degrees C for fish acclimated to 15 degrees C) by performing continuous infusions of 6-[3H]glucose and 1-[14C]palmitate in vivo. Results show that hypoxia causes a 53% decrease in NEFA turnover rate, together with a transient increase in hepatic glucose production, whereas a rapid drop in temperature induces equivalent declines in glucose, NEFA, and oxygen fluxes [temperature coefficient approximately equal to 2]. More importantly, kinetic changes in glucose and NEFA fluxes are not accompanied by interpretable changes in the plasma concentrations of these metabolites. Thus using concentration changes to draw conclusions about fluxes must be avoided.

References

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Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J W WojcieszynK A Jacobson
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Jan 1, 1989·Fish Physiology and Biochemistry·J F Steffensen

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Citations

Apr 25, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Leonardo MagnoniJean-Michel Weber
Jan 1, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kevin Choi, Jean-Michel Weber
Jan 31, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Johnathon L I ForbesJean-Michel Weber
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J M Weber, D S Shanghavi
Oct 30, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ben Speers-RoeschJeffrey G Richards
Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Teye OmlinJean-Michel Weber
Nov 27, 2019·The Journal of Experimental Biology·Johnathon L I ForbesJean-Michel Weber
Jun 26, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Eric Vaillancourt, Jean-Michel Weber
Nov 6, 2007·The Journal of Experimental Biology·Leonardo Magnoni, Jean-Michel Weber
Jul 16, 2008·The Journal of Experimental Biology·Leonardo MagnoniJean-Michel Weber
Nov 3, 2010·The Journal of Experimental Biology·Teye Omlin, Jean-Michel Weber
Jan 7, 2005·The Journal of Experimental Biology·Eric VaillancourtJean-Michel Weber
May 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M L Figueiredo-GaruttiM L M Vicentini-Paulino
Jul 23, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ivan ViegasJohn G Jones

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