PMID: 15374471Nov 1, 1990Paper

Effects of idebenone on the composition of brain synaptosomal membranes of rats

Archives of Gerontology and Geriatrics
K NagyI Zs -Nagy

Abstract

Male and female CFY rats of 21 months of age were treated per os by 50 mg idebenone per kg body weight/day for 5 weeks while the controls received only the solvent. Synaptosomal fractions of the brain cortex were prepared and tested as follows: (1) Labeling by 1,6-diphenyl-1,3,5-hexatriene (DPH) and the fluorescence anisotropy (r) of this label was measured at various optical densities. The average values of r extrapolated to zero optical density were 0.1831 +/- 0.0025 in the control group and 0.1854 +/- 0.0019 in the idebenone-treated animals; the difference is statistically not significant. (2) Labeling with 3 different spin labels (5-NS, N-oxyl-4', 4'-dimethyloxazolidine derivative of stearic acid with the doxyl groups in C-5; 16-NS, the same derivative of stearic acid with the doxyl groups in C-16 position; and MAL-SL, 3-maleimido-2,2,5,5-tetramethyl-1-pyrrolidinyloxy). Routine ESR spectroscopy was applied to characterize the molecular motion of these labels in their microenvironment. These spin labels did not display any significant change in their environment under the influence of idebenone treatment. (3) Protein contents of the samples were measured and samples of synaptosomes containing 3.5 mg protein were dissolved in...Continue Reading

References

Nov 1, 1976·Archives of Biochemistry and Biophysics·D A ButterfieldD B Chesnut
Oct 1, 1978·Archives of Biochemistry and Biophysics·G L Jones, D M Woodbury
Apr 16, 1976·Biochimica Et Biophysica Acta·J A BabitchD L Helseth
Jan 27, 1971·Journal of the American Chemical Society·W L Hubbell, H M McConnell
Dec 28, 1983·Biochemical and Biophysical Research Communications·K NagyI Zs-Nagy
Nov 1, 1990·Archives of Gerontology and Geriatrics·I Zs -Nagy
Nov 1, 1990·Archives of Gerontology and Geriatrics·I Zs -Nagy, R A Floyd

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