Effects of in vivo treatments of nicotine and benzo[a]pyrene on the epidermal growth factor receptor in hamster buccal pouch

Toxicology
S L WangC Y Wu-Wang

Abstract

This study was conducted to investigate the effects of two major ingredients in cigarette smoke, benzo-alpha-pyrene (BP) and nicotine (Nic), on epidermal growth factor (EGF) receptor in hamster buccal pouch. Adult male Syrian hamsters were treated by apically swabbing the buccal pouch with mineral oil (Control), 0.5 microgram/ml of BP, Nic or BP+Nic in mineral oil, twice a day, 5 days a week for 4 weeks. The BP+Nic treatment resulted in a significant reduction of submandibular gland (SG) EGF (Control vs. BP+Nic, 6.93 +/- 1.31 vs. 4.77 +/- 0.26* ng/g wet tissue, mean +/- S.D., n=5,*P < 0.05). Treatment with BP or Nic also caused a reduction, although not statistically significant, of EGF in SG extract. For the receptor study, all treatments significantly increased [125I]EGF binding to membrane preparations of buccal pouch as compared to Control (Control, BP, Nic, BP+Nic; 12.2 +/- 0.9, 20.5 +/- 2.2*, 17.0 +/- 1.3*, 21.2 +/= 1.6* fmol/mg prot. (mean +/- S.E.M.), n=5, P < 0.05). Scatchard analysis revealed that the higher EGF binding to the BP+Nic-treated sample was due to the higher number of receptors, but not higher affinity. Data from protein kinase study indicate that EGF stimulated phosphorylation of 170- and 150-kDa proteins...Continue Reading

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Citations

Jul 6, 2000·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·J NovakM A Kane
Apr 30, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Shuo WangYoichi Nakanishi
Oct 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Yi N YeChi H Cho
May 1, 1999·Alternatives to Laboratory Animals : ATLA·G CiapettiL Checchi

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