Abstract
Different times of incomplete cerebral ischemia (2, 4, 6, 8, 10 and 30 min) were induced by bilateral common carotid artery occlusion in anesthetized rats to evaluate the time course of changes in lipid peroxidation and energy metabolism. Analysis of malondialdehyde (used to assess the levels of lipid peroxidation), ascorbic acid, oxypurines, nucleosides, nicotinic coenzymes and high-energy phosphates, was carried out by high-performance liquid chromatography on neutralized perchloric acid extract of brain tissue. Under the present experimental conditions, malondialdehyde, nicotinic coenzymes and ATP catabolites (oxypurines and nucleosides) were affected by increasing times of ischemia, with respect to control sham-operated rats. In particular, the concentration of malondialdehyde, undetectable in control brains, increased from 1.26 nmol/g wet weight after 2 min of carotid clamping to 13.42 nmol/g wet weight at the end of 30 min of incomplete cerebral ischemia. The presence of oxidative stress was further supported by ascorbic acid depletion, which was particularly significant after 10 and 30 min of incomplete ischemia. Carotid clamping provoked an imbalance between energy production and consumption that was evidenced by a redu...Continue Reading
Citations
Jan 7, 2004·Analytical Biochemistry·Giuseppe LazzarinoBarbara Tavazzi
Dec 23, 2008·Brain Research·Kun LiuLanqun Mao
Oct 8, 1999·Journal of Neuroscience Methods·K L Clapp-LillyK L Drew
Oct 28, 1999·Neurobiology of Aging·C D LynchW E Sonntag
Nov 5, 1999·Journal of Neurotrauma·R VagnozziG Lazzarino
Nov 19, 2011·Multiple Sclerosis International·Barbara TavazziGiuseppe Lazzarino
Nov 3, 2010·Neurosurgical Focus·Stefano SignorettiGiuseppe Lazzarino
Aug 31, 2000·Analytical Biochemistry·D Di PierroR Giuffrè
Dec 28, 1999·Analytical Biochemistry·B TavazziG Lazzarino
Aug 3, 2001·Journal of Neurochemistry·C LuM P Mattson
Apr 4, 2009·Clinical Biochemistry·Angela M AmoriniGiuseppe Lazzarino
Dec 6, 2008·Brain Research·Evelin VicenteCarlos Alberto Gonçalves
Feb 26, 2008·Neurochemistry International·Kun LiuLanqun Mao
Jul 19, 2005·Brain Research·Rainald Schmidt-KastnerMyron D Ginsberg
Aug 25, 2012·Neuroscience·B RocamondeJ M Soria
Nov 9, 2016·Molecular Neurobiology·Giacomo LazzarinoBarbara Tavazzi
Feb 26, 2005·Neurosurgery·Barbara TavazziRoberto Vagnozzi
Mar 21, 2012·The Journal of International Medical Research·M T GokdemirM E Boleken
Oct 25, 2001·The Journal of Biological Chemistry·B TavazziG Lazzarino
May 29, 2000·Free Radical Research·B TavazziG Lazzarino