Effects of increasing times of incomplete cerebral ischemia upon the energy state and lipid peroxidation in the rat

Experimental Brain Research
Roberto VagnozziGiuseppe Lazzarino

Abstract

Different times of incomplete cerebral ischemia (2, 4, 6, 8, 10 and 30 min) were induced by bilateral common carotid artery occlusion in anesthetized rats to evaluate the time course of changes in lipid peroxidation and energy metabolism. Analysis of malondialdehyde (used to assess the levels of lipid peroxidation), ascorbic acid, oxypurines, nucleosides, nicotinic coenzymes and high-energy phosphates, was carried out by high-performance liquid chromatography on neutralized perchloric acid extract of brain tissue. Under the present experimental conditions, malondialdehyde, nicotinic coenzymes and ATP catabolites (oxypurines and nucleosides) were affected by increasing times of ischemia, with respect to control sham-operated rats. In particular, the concentration of malondialdehyde, undetectable in control brains, increased from 1.26 nmol/g wet weight after 2 min of carotid clamping to 13.42 nmol/g wet weight at the end of 30 min of incomplete cerebral ischemia. The presence of oxidative stress was further supported by ascorbic acid depletion, which was particularly significant after 10 and 30 min of incomplete ischemia. Carotid clamping provoked an imbalance between energy production and consumption that was evidenced by a redu...Continue Reading

Citations

Jan 7, 2004·Analytical Biochemistry·Giuseppe LazzarinoBarbara Tavazzi
Oct 8, 1999·Journal of Neuroscience Methods·K L Clapp-LillyK L Drew
Nov 19, 2011·Multiple Sclerosis International·Barbara TavazziGiuseppe Lazzarino
Apr 4, 2009·Clinical Biochemistry·Angela M AmoriniGiuseppe Lazzarino
Dec 6, 2008·Brain Research·Evelin VicenteCarlos Alberto Gonçalves
Mar 21, 2012·The Journal of International Medical Research·M T GokdemirM E Boleken

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