Effects of incubation time and buffer concentration on in vitro activities of antifungal agents against Candida albicans.

Journal of Clinical Microbiology
M A TornatoreL R Peterson

Abstract

Nine selected isolates of Candida albicans were tested for their susceptibilities to amphotericin B and fluconazole by using three methods to assess the effect of incubation time and buffer concentration. By using a microdilution method with 0.0165 M 3-(N-morpholino)propanesulfonic acid (MOPS) and a 24-h incubation time, all of the isolates were found to be susceptible to amphotericin B and fluconazole. After 48 h of incubation, all isolates were still susceptible to amphotericin B. Seven of the nine isolates were resistant to fluconazole, and for the remaining two isolates, MICs increased by fourfold or more but the isolates remained susceptible (MIC, < or = 10 microg/ml). The nine isolates, along with three control strains, were further tested against amphotericin B and fluconazole by a standard broth macrodilution method with both 0.165 and 0.0165 M MOPS. The susceptibility results for fluconazole by the broth macrodilution method with the lower MOPS concentration correlated with the results of the 24-h broth microdilution method for determination of susceptibility or resistance in eight of nine tests and with the results of the 48 h broth microdilution method in three of nine tests. The results of the broth macrodilution me...Continue Reading

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Citations

Nov 20, 2009·Journal of Applied Microbiology·J R SoberónM A Vattuone
Nov 16, 2011·Chemistry & Biodiversity·Gökalp IşcanK Hüsnü Can Başer
Oct 5, 2001·Clinical Microbiology Reviews·J H RexD W Warnock
Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Marcos Dornelas-RibeiroSergio Eduardo Longo Fracalanzza
Aug 15, 2001·Pharmacotherapy·H L Hoffman, M A Pfaller
May 29, 1999·Antimicrobial Agents and Chemotherapy·K A MarrT C White
Feb 7, 2001·Journal of Clinical Microbiology·M Cuenca-EstrellaJ L Rodríguez-Tudela
Aug 30, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Cuenca-Estrella, J L Rodríguez-Tudela

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