Effects of Indocyanine green on cultured retinal ganglion cells in-vitro.

BMC Research Notes
S BalaiyaKv Chalam

Abstract

Indocyanine green (ICG) dye is commonly used to stain the inner limiting membrane during macular surgery. There are reports documenting the toxicity of ICG on retinal pigment epithelial cells, with conflicting results in retinal ganglion cells. In the present study, we evaluated the effect of ICG on retinal ganglion cells in vitro.Cultured rat retinal ganglion cells (RGC-5) were exposed to different concentrations of ICG (0.25, 0.5, 1.0, 1.25, & 5 mg/ml) and at various time intervals (1, 5, 15, 30, & 60 minutes). Changes in structural morphology were identified using phase contrast bright field microscopy. Cell viability was quantified using the neutral red assay and cell death was characterized using Annexin-V staining. Significant morphologic changes were observed at the 15 and 60 min intervals for all concentrations, where a reduction in cell size and loss of normal spindle shape was noted. A dose dependent decrease in cell viability was observed with increasing concentration of ICG as well as increasing exposure intervals. Compared to control, 48-74% reduction in neutral red uptake at all concentrations for exposures 5 min or greater (p < 0.001). Even at 1 min exposure, a dose dependent decline was observed in cell viabilit...Continue Reading

References

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Citations

Aug 24, 2010·American Journal of Ophthalmology·Srinivas AmbatipudiK V Chalam
Apr 2, 2014·Biomedical Optics Express·Beata ToczylowskaAdam Liebert
Mar 21, 2017·The British Journal of Ophthalmology·Xiao-Ling FangZhao-Yang Wang
Aug 20, 2016·Optica·Yu-Cheng ChenXudong Fan

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Methods Mentioned

BETA
Flow
flow cytometry
Assay

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Graphpad Prizm

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