Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells

The Journal of Physiology
M J AndersonE Zorychta

Abstract

1. Myotomal muscle cells from embryos of Xenopus laevis were cultured as a monolayer either alone or together with neural tube cells from the same embryos. 2. Spontaneous twitching and contractions evoked by electrical stimulation of neural perikarya were observed only in nerve-contacted muscle cells, and could be abolished by curare or alpha-bungarotoxin. 3. Within 2 days in culture muscle cells not contacted by nerve developed one or more discrete patches of acetylcholine (ACh) receptors as revealed by staining with fluorescent conjugates of alpha-bungarotoxin. Similar patches were also seen when staining was carried out after paraformaldehyde fixation, suggesting that they were not induced by the dyetoxin conjugate. 4. Radioautography after labelling with [125I]alpha-bungarotoxin revealed patches with grain densities approximately twenty-five-old greater than over the remainder of the cell. 5. Fluorescent stain on innervated cells was restricted to the path of nerve-muscle contact and sometimes extended for greater lengths than the largest patches seen on non-contacted muscle cells. 6. Similar long bands of stain associated with nerve--muscle contacts were observed when cultures were grown in high concentrations of curare an...Continue Reading

Citations

Jan 1, 1983·The Journal of Membrane Biology·D Axelrod
Jan 1, 1980·Pharmacology & Therapeutics·A L Harvey
Jan 1, 1989·Molecular Neurobiology·R Laufer, J P Changeux
Nov 4, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J M HackettM Griffith
Feb 6, 2007·Computer Methods and Programs in Biomedicine·Kok-Yong SengPaolo Vicini
May 1, 1983·Developmental Biology·J R Sanes, J C Lawrence
Jun 3, 2008·Chemico-biological Interactions·Lin GengH Benjamin Peng

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