PMID: 3319619Dec 30, 1987Paper

Effects of insulin and norepinephrine on glucose transport and metabolism in rat brown adipocytes. Potentiation by insulin of norepinephrine-induced glucose oxidation

European Journal of Biochemistry
S EbnerJ Girard

Abstract

Glucose is an important fuel for rat brown adipose tissue in vivo and its utilization is highly sensitive to insulin. In this study, the different glucose metabolic pathways and their regulation by insulin and norepinephrine were examined in isolated rat brown adipocytes, using [6-14C]glucose as a tracer. Glucose utilization was stimulated for insulin concentrations in the range of 40-1000 microU/ml. Furthermore, the addition of adenosine deaminase (200 mU/ml) or adenosine (10 microM) did not alter insulin sensitivity of glucose metabolism. The major effect of insulin (1 mU/ml) was a respective 7-fold and 5-fold stimulation of lipogenesis and lactate synthesis, whereas glucose oxidation remained very low. The 5-fold stimulation of total glucose metabolism by 1 mU/ml of insulin was accompanied by an 8-fold increase in glucose transport. In the presence of norepinephrine (8 microM), total glucose metabolism was increased 2-fold. This was linked to a 7-fold increase of glucose oxidation, whereas lipogenesis was greatly inhibited (by 72%). In addition, norepinephrine alone did not modify glucose transport. The addition of insulin to adipocytes incubated with norepinephrine, induced a potentiation of glucose oxidation, while lipogen...Continue Reading

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Citations

Dec 25, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Sunhee KimChun K Kim
May 26, 2016·European Journal of Endocrinology·Moe Thuzar, Ken K Y Ho
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Oct 1, 1989·The American Journal of Physiology·A Marette, L J Bukowiecki
Jan 1, 1989·Progress in Lipid Research·J Himms-Hagen

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