Effects of insulin-like growth factor-1 on endoplasmic reticulum stress and autophagy in rat gastric smooth muscle cells cultured at different glucose concentrations in vitro

Molecular and Cellular Biochemistry
Xue-Sen FangZheng Jin

Abstract

The purpose of the study was to observe changes in endoplasmic reticulum stress (ERS)- and autophagy-related proteins in gastric smooth muscle tissues of diabetic rats with gastroparesis, investigate the effect of insulin-like growth factor 1 (IGF-1) on ERS and autophagy in rat gastric smooth muscle cells cultured under different glucose concentrations, and explore the influence of IGF-1 on development of diabetic gastroparesis (DGP). After establishing a rat model of DGP, rats were divided into normal control (NC) and 6-week diabetic model (DM6W) groups. Expression of ERS-related and autophagy-related proteins was detected by western blot analysis and immunofluorescence assay in rat gastric smooth muscle tissue and in vitro-cultured rat gastric smooth muscle cells exposed to different glucose concentrations and treatment with IGF-1 for 24 or 48 h. Changes in glucose-regulated-protein-78 (GRP78), growth arrest and DNA damage-inducible gene 153 (CHOP), and microtubule-associated protein 1A/1B light chain 3B (LC3) expression levels were detected by western blot analysis, and GRP78 and LC3 expression were examined by confocal laser-scanning microscopy. In vivo expression levels of GRP78, CHOP, and LC3 were significantly higher in ...Continue Reading

References

Jan 22, 2002·Best Practice & Research. Clinical Endocrinology & Metabolism·P V Carroll
Jun 15, 2007·Nature Reviews. Molecular Cell Biology·David Ron, Peter Walter
Aug 12, 2010·Methods in Molecular Biology·Martin Schröder, Louise Sutcliffe
Sep 4, 2010·Circulation Research·Sarah Hummasti, Gökhan S Hotamisligil
Sep 6, 2011·Trends in Molecular Medicine·Miriam CnopLicio A Velloso
Mar 17, 2012·Journal of Cellular Physiology·Kyle T PfaffenbachAmy S Lee
Jul 11, 2014·World Journal of Gastroenterology : WJG·Xia ChenHong-Xian Zhao
Nov 26, 2014·Nature Reviews. Endocrinology·Liza K PhillipsMichael Horowitz
Jun 3, 2015·World Journal of Gastroenterology : WJG·Hong-Liang Wang, Kai-Jiang Yu
Oct 25, 2016·Chemico-biological Interactions·Ali RoohbakhshGholamreza Karimi
May 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yang Zhou, Wei Wu
May 27, 2017·Osteoarthritis and Cartilage·S A NazliR R Yammani
Oct 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Fan YangFanghao Lu

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

GraphPad
IPP6
GraphPad Prism5
Image Pro Plus

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