Effects of integration host factor and DNA supercoiling on transcription from the ilvPG promoter of Escherichia coli.

The Journal of Biological Chemistry
B S ParekhG W Hatfield

Abstract

Integration host factor (IHF) activates transcription from the ilvPG promoter by severely distorting the DNA helix in an upstream region of a supercoiled DNA template in a way that alters the structure of the DNA in the downstream promoter region and facilitates open complex formation. In this report, the in vivo and in vitro influence of DNA supercoiling on transcription from this promoter is examined. In the absence of IHF, promoter activity increases with increased DNA supercoiling. In the presence of IHF, the same increases in superhelical DNA densities result in larger increases in promoter activity until a maximal activation of 5-fold is obtained. However, the relative transcriptional activities of the promoter in the presence and absence of IHF at any given DNA superhelical density remains the same. Thus, IHF and increased DNA supercoiling activate transcription by different mechanisms. Also, IHF binds with equal affinities to its target site on linear and supercoiled DNA templates. Therefore, IHF binding does not activate transcription simply by increasing the local negative supercoiling of the DNA helix in the downstream promoter region or by differential binding to relaxed and supercoiled DNA templates.

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Citations

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May 14, 1999·Current Opinion in Microbiology·X Dai, L B Rothman-Denes
May 22, 2001·Molecular Microbiology·S D SheridanG W Hatfield
Jan 19, 1999·Annual Review of Microbiology·A J Cozzone
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·K Y RheeG W Hatfield
Nov 14, 2002·Annual Review of Genetics·G Wesley Hatfield, Craig J Benham
Nov 19, 2013·PLoS Genetics·Rudra P SahaRasika M Harshey
Jan 1, 1997·Biopolymers·J M SchurrA S Benight
Mar 7, 2007·Science Progress·Charles J Dorman
Jun 7, 2019·The Journal of Biological Chemistry·Pierre PlateauSylvain Blanquet
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