Effects of interferon-alpha on cytokine profile, T cell receptor repertoire and peptide reactivity of human allergen-specific T cells

European Journal of Immunology
P ParronchiS Romagnani

Abstract

A large panel of T cell clones (TCC) specific for the recombinant form of Poa pratensis allergen (rKBG7.2 or Poa p9) were established from the peripheral blood of grass pollen-sensitive donor in the absence or presence of recombinant interferon-alpha (IFN-alpha) in bulk culture and their pattern of cytokine secretion, peptide reactivity and TCR V beta repertoire was examined. The majority of allergen-specific TCC derived in absence of IFN-alpha produced high amounts of interleukin-4 (IL-4) and IL-5 but not IFN-gamma (Th2 cells), while most of TCC derived in presence of IFN-alpha produced IFN-gamma but not, or limited amounts of, IL-4 and IL-5 (Th1 or Th0 cells). Of 24 TCC established in the presence of IFN-alpha, 22 were able to recognize a single allergen peptide, p26, while none of the clones established in the absence of IFN-alpha showed a similar specificity. The majority of both clones expressed the V beta 2 element regardless of whether they were established in the presence of INF-alpha, but the presence of IFN-alpha favored the expansion of V beta 2+, V beta 17+ and V beta 22+ Poa p9-specific T cells, whereas in the absence of IFN-alpha, other TCR V beta-bearing T cells (V beta 5, and V beta 6.7, and V beta 14) were expa...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 30, 1992·The New England Journal of Medicine·D S RobinsonA B Kay
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P ParronchiS Romagnani
Jan 1, 1991·Research in Immunology·S L Swain
Jul 1, 1988·European Journal of Immunology·E MaggiS Romagnani
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PèneJ Banchereau
Apr 1, 1995·The Journal of Experimental Medicine·C PfeifferK Bottomly
Dec 1, 1994·Current Opinion in Immunology·S Romagnani
Dec 1, 1994·European Journal of Immunology·S G NadlerP A Kiener
Jan 1, 1994·Annual Review of Immunology·S Romagnani
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C Howard
Mar 5, 1993·Science·S L ReinerR M Locksley
Apr 16, 1993·Science·L C HsiehP G Schultz

❮ Previous
Next ❯

Citations

Jan 1, 1996·International Journal of Clinical & Laboratory Research·S Romagnani
May 16, 2007·Cancer Immunology, Immunotherapy : CII·Jason M ZimmererWilliam E Carson
Nov 27, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Deshka DoychevaManfred Zierhut
Mar 23, 2004·Journal of the American Academy of Dermatology·Cindy J RogersVladimir Vincek
Dec 1, 1999·Developmental and Comparative Immunology·F SinigagliaL Rogge
Jan 19, 2000·Immunology Today·C L KarpD N Irani
Jul 22, 1998·Journal of Hepatology·R M HoffmannG R Pape
Jul 19, 2000·Current Opinion in Immunology·C Bogdan
Apr 8, 1998·Immunotechnology : an International Journal of Immunological Engineering·E Maggi
Jul 10, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·K J SmithH Skelton
Dec 20, 2000·The Journal of Experimental Medicine·J BanchereauK Palucka
Jul 1, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E J BartholoméM Goldman
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·W TuoW C Brown
Aug 20, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Christine Rogez-KreuzPascal Clayette
Jan 31, 2002·AIDS·F P Siegal, G T Spear
Mar 11, 2005·Medicine·Manuel Ramos-CasalsUNKNOWN HISPAMEC Study Group
Jan 1, 1997·Journal of Viral Hepatitis·F Balkwill
Mar 1, 1997·Allergy·A Ruffilli, S Bonini
Mar 3, 1997·The Journal of Experimental Medicine·L RoggeF Sinigaglia
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·D M EssayanS K Huang
Jul 25, 2000·Clinics in Chest Medicine·C Ober, M F Moffatt
Jan 1, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Armin LuikJörg F Schlaak
Mar 2, 2011·The American Journal of Pathology·Stefan ProkopWerner Stenzel
Jun 25, 2010·Current Opinion in Pharmacology·Shyam S MohapatraGary Hellermann
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J J LasarteJ Prieto
Mar 23, 2001·Nature Immunology·S Patterson
Sep 18, 2004·Clinical and Experimental Immunology·U SchauerV Stephan
Dec 7, 2006·Journal of Leukocyte Biology·Adriana A ByrnesChristopher L Karp
Oct 26, 2006·Journal of the European Academy of Dermatology and Venereology : JEADV·A Alonso-PérezJ Fernández-Herrera
Sep 23, 2009·Melanoma Research·Carlos Suárez-GarcíaFrancisco Miguel Camacho
Sep 14, 2013·European Journal of Immunology·Philippa HillyerRonald L Rabin
May 24, 2001·Current Opinion in Neurology·C L KarpL Nagelkerken
Dec 31, 1998·Springer Seminars in Immunopathology·A Faro
Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jui-Hung YenDoina Ganea

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