Effects of interferon-gamma reverse hypoxia-stimulated extracellular matrix expression in human peritoneal and adhesion fibroblasts

Fertility and Sterility
Ghassan M Saed, Michael P Diamond

Abstract

To determine the response of adhesion and normal peritoneal fibroblasts to interferon-gamma (IFN-gamma) under normal and hypoxic conditions. Prospective experimental study. University medical center. Primary cultures of fibroblasts established from peritoneal and adhesion tissue of the same patients. Hypoxia and IFN-gamma treatment of the primary cultured fibroblasts. Primary cultures of fibroblasts were established from peritoneal and adhesion tissues of the same patients (n = 5). The expression of extracellular matrix components (type I collagen and fibronectin) in adhesion and peritoneal fibroblasts under normal (20% O2) and hypoxic (2% O2) conditions was evaluated by multiplex reverse-transcription polymerase chain reaction analysis. Adhesion fibroblasts (ADF) have increased basal levels of type I collagen as compared with normal peritoneal fibroblasts (NF). Interferon-gamma treatment resulted in a dose-response decrease in type I collagen and fibronectin mRNA levels in both ADF and NF. Hypoxia treatment resulted in a time-response increase in type I collagen and fibronectin mRNA levels in NF and ADF. Hypoxia had no effect on type I collagen and fibronectin mRNA levels in the presence of increasing dose of IFN-gamma in both...Continue Reading

Citations

May 4, 2015·Human Reproduction Update·Chelsea N FortinMichael P Diamond
Feb 28, 2016·Fertility and Sterility·Nicole M FletcherGhassan M Saed
Feb 13, 2014·Reproductive Sciences·Awoniyi O AwonugaGhassan M Saed
Mar 3, 2010·The British Journal of Dermatology·J P McFaddenL Fry
Dec 17, 2008·Annals of the New York Academy of Sciences·Karen L ReedJames M Becker
Oct 23, 2020·Reproductive Sciences·Mili ThakurAwoniyi O Awonuga

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