PMID: 2509133Jan 1, 1989Paper

Effects of interleukin-1 and lipopolysaccharides on protein and carbohydrate metabolism in bovine articular cartilage organ cultures

Connective Tissue Research
T I Morales, V C Hascall

Abstract

The long term (18 day) metabolic response of bovine articular cartilage to treatment with either E. Coli lipopolysaccharide (LPS) or interleukin 1 was studied. For LPS treatment, incorporation of [35S]sulfate into the large proteoglycan population was inhibited 80% while that into the small interstitial proteoglycans was only inhibited 40%. Incorporation of [3H]serine into the large proteoglycan population was inhibited approximately 72% while incorporation into other protein was inhibited only 16%. Furthermore, the rate of catabolism of [3H]serine labeled proteoglycans was increased 2-fold by LPS treatment while the rate of 3H-labeled general protein catabolism was not affected. Incorporation of [3H]glucosamine into hyaluronate was increased; however a correction for changes in the specific activity of the intracellular [3H]glucosamine precursor pool in LPS-treated cultures indicated that the net amount of hyaluronate synthesized was not altered by LPS treatment. The 3H/35S ratios in isolated chondroitin sulfate disaccharides labeled with [35S]sulfate and [3H]glucosamine precursors were significantly changed during long term LPS treatment, suggesting that general carbohydrate pathways are altered. The 3H/35S changes were large...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L M WahlS E Mergenhagen
Dec 30, 1986·Biochemical and Biophysical Research Communications·F OllivierreB V Treadwell
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E R PettipherB Henderson
May 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A R PooleL C Rosenberg
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jan 1, 1984·Reviews of Infectious Diseases·C A Dinarello
Jul 1, 1983·Archives of Biochemistry and Biophysics·V C HascallD A Lowther

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Citations

Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J HoranB D Boyan
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D D DeanD S Howell
Feb 1, 1997·Arthritis Care and Research : the Official Journal of the Arthritis Health Professions Association·J C BautchA C Vailas
Dec 17, 2014·Life Sciences·Samir M AbdelmagidFayez F Safadi
Oct 15, 1992·Biochemical and Biophysical Research Communications·R M PalmerS Moncada
Oct 1, 1989·Arthritis and Rheumatism·T I Morales, V C Hascall
Jan 13, 2006·Osteoarthritis and Cartilage·M C StewartJ D Sandy
Mar 1, 1997·Osteoarthritis and Cartilage·D MargerieE Bartnik
Sep 28, 2014·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Jack FarrJames L Cook
Nov 1, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carrie H-Y LingMarc E Levenston
Sep 26, 2013·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·A Von KeudellA H Gomoll
Sep 4, 2003·The Journal of Biological Chemistry·Paul J FanningStephen B Trippel
Jan 23, 1997·Biochemical and Biophysical Research Communications·P A ScherleE C Arner
Nov 26, 1997·Antimicrobial Agents and Chemotherapy·M MenschikW B Graninger

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