Effects of interleukin-1 beta on thyrotropin secretion and thyroid hormone uptake in cultured rat anterior pituitary cells

Endocrinology
F W WassenMaria E Everts

Abstract

The effects of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF alpha) on basal and TRH-induced TSH release, and the effects of IL-1 beta on the uptake of [125I]T3 and [125I]T4 and on nuclear binding of [125I]T3 were examined. Furthermore, the release of other anterior pituitary hormones in the presence of IL-1 beta was measured. Anterior pituitary cells from male Wistar rats were cultured for 3 days in medium containing 10% FCS. Incubation were performed at 37 C in medium with 0.5% BSA for measurement of [125I]T3 uptake and with 0.1% BSA for measurement of [125I]T4 uptake. Exposure to IL-1 beta (1 pM-1 nM) or TNF alpha (100 pM) for 2-4 h resulted in a significant decline in TSH release, which was almost 50% (P < 0.05) for 1 nM IL-1 beta and 24% (P < 0.05) for 100 pM TNF alpha. Measurement of other anterior pituitary hormones (FSH, LH, PRL, and ACTH) in the same incubation medium showed that IL-1 beta did not alter their release. When the effects of IL-1 beta (1 pM-1 nM) and TNF alpha (100 pM) on TRH-induced TSH release were measured in short term experiments, the inhibitory effects had disappeared. The addition of 1-100 nM octreotide, a somatostatin analog, resulted in a decrease in TRH-induced TSH release u...Continue Reading

Citations

Apr 1, 1997·Endocrine Reviews·D Ray, S Melmed
Jul 26, 2019·Current Opinion in Endocrinology, Diabetes, and Obesity·An JacobsGreet Van den Berghe
Jun 14, 2020·International Journal of Molecular Sciences·Maciej WójcikAgata Krawczyńska
Feb 10, 2021·Journal of Endocrinological Investigation·L CroceL Chiovato
Jun 20, 2020·Frontiers in Neuroendocrinology·Pedro MarquesMárta Korbonits
Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·K M O'ConnorF B McDonald

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