Effects of interleukin-1, lipopolysaccharide, and streptococci on procoagulant activity of cultured human cardiac valve endothelial and stromal cells.

Infection and Immunity
T A Drake, M Pang

Abstract

Fibrin is the primary constituent of the vegetation in infective endocarditis, and tissue factor expression is a major mechanism of coagulation activation on infected valves. To determine which cells may participate in coagulation activation in this setting, expression of procoagulant activity (PCA; shown to be tissue factor) was studied in cultured endothelial and stromal cells derived from human cardiac valves. Endothelial cells had negligible PCA (99 +/- 50 mU/10(5) cells, mean +/- 1 standard deviation) unless stimulated by lipopolysaccharide or interleukin-1, which increased PCA to 5,592 +/- 1,482 and 5,901 +/- 1,497 mU/10(5) cells, respectively, in 6 h. Incubation of cells with viable enterococci or viridans streptococci or with an enterococcal cell wall preparation did not induce PCA. Cultured valve stromal cells constitutively expressed high levels of PCA (14,276 +/- 8,738 mU/10(5) cells) which was not changed with exposure to interleukin-1. PCAs of stromal or stimulated endothelial cells from valves of both right and left sides of the heart were comparable. The results suggest that endothelial cells may contribute to fibrin deposition during infection if stimulated, but PCA is not directly induced by bacteria. Stromal c...Continue Reading

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Citations

Feb 26, 2015·Journal of Tissue Engineering and Regenerative Medicine·Soumen JanaAmir Lerman
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·J R ToomeyG J Broze
Nov 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H FeiT A Drake
Jun 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jonathan T Butcher, Robert M Nerem
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Mar 1, 1997·Infection and Immunity·M R YeamanM E Selsted

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