PMID: 12781029Jun 5, 2003Paper

Effects of interleukin-18 on asthmatic airway inflammation and nuclear factor kappa-B in murine models

Chinese Medical Journal
Huilan ZhangYongjian Xu

Abstract

To investigate the effects of Interleukin-18 (IL-18) on asthmatic airway inflammation and nuclear factor kappa-B (NF-kappaB) in a murine asthmatic model. BALB/C mice were randomly divided into three groups: group A (control group, n = 10); group B (asthmatic model group, n = 10); group C (IL-18 injection group, n = 10). The asthmatic model was established in group B and C by respiratory syncytial virus (RSV) killed by ultraviolet light. Saline solution (0.1 ml) and IL-18 (0.1 ml, 1 micro g) was injected in groups B and C at seven time points (1, 2, 7, 8, 9, 21, 22 d). The symptoms and the numbers of eosinophils and plasmacytes in the airways were observed and the expression of NF-kappaB activation in the lung was analyzed by immunohistochemistry (IHC) and Western blot. The symptoms of group C were more severe than in groups A and B. Group A did not have EOS and plasmacytes in the airway submucosal while the numbers of eosinophils [15 +/- 3 (average cell counts per microscopic visual field, the same below)] and plasmacytes (10 +/- 2) in group B were found to have increased significantly. But the numbers of eosinophils and plasmacytes in group C were decreased significantly when compared with group B (6 +/- 2 and 2 +/- 1, respect...Continue Reading

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.