Effects of interleukin-2 and interleukin-2-activated cells on in vitro myelopoiesis

Clinical and Experimental Immunology
M ClerigueM Hanson

Abstract

Lymphokine-activated killer (LAK) cells from human peripheral blood mononuclear cells cultured with recombinant interleukin-2 (IL-2) have been used clinically in adoptive immunotherapy for cancer patients. To study the influence of LAK cells and IL-2 on haematopoiesis, an in vitro assay system for colony formation of granulocyte-macrophage progenitor cells (GM-CFC) was used. LAK cells from cultures of either human peripheral blood (PB) or human bone marrow (BM) mononuclear cells were both inhibitory to allogeneic BM-derived GM-CFC. Inhibitory activity could be transferred with supernatants from co-cultures of LAK cells and BM targets, but also from the IL-2 activated PB- or BM-derived cells alone. The inhibitory activity from the initially non-cytotoxic/non-inhibitory BM population was rapidly induced by IL-2 activation, and preceded the generation of cytotoxic LAK cells in the culture. These experiments show that inhibition of haematopoietic progenitor cells by IL-2 is not dependent on generation of cytotoxic LAK cells, but rather the result of IL-2-induced cytokine production. We conclude that the synergistic action of interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) may contribute to inhibition, but ...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H SaitoT Ishizaka
Mar 1, 1988·European Journal of Immunology·M HanssonR Kiessling
Aug 1, 1970·Journal of Cellular Physiology·B L Pike, W A Robinson
Jan 1, 1981·European Journal of Immunology·M HanssonB Andersson
Jan 1, 1982·Journal of Immunological Methods·T TimonenR B Herberman

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