Abstract
Among conventional osteoarthritis (OA) treatments, intra-articular (i.a) viscosupplementation with hyaluronic acid (HA) is used to restore joint viscoelasticity. However, the rapid clearance and elimination of HA may limit its application. The aim of this study was to verify the improved efficacy of HA within the joint, using a lactose-modified chitosan (chitlac) as a potentially chondroprotective additive. Four weeks after induction of experimental OA by destabilization of the medial meniscus (DMM), 12-week-old Sprague Dawley male rats (n = 30), received once a week, for three weeks, i.a injections of: (i) HA associated to chitlac (ARTY-DUO®), (ii) HA; and (iii) sodium chloride (NaCl). Five animals for each group were euthanized 4 weeks after the first i.a injection, while the remaining five were euthanized 8 weeks after the first i.a injection. The restoration of physiological joint microenvironment was tested by histology, histomorphometry, immunohistochemistry, and microtomography (micro-CT). At 4 and even more at 8 weeks, histological analysis showed a significant decrease in OARSI and Mankin scores, with weaker matrix metalloproteinase (MMP)-3, MMP-13, and Galectin-3 in ARTY-DUO® group versus NaCl and HA groups. A reducti...Continue Reading
References
Apr 1, 2000·Carbohydrate Research·N KubotaK Toya
Jul 6, 2000·Journal of Biomedical Materials Research·A LahijiC G Frondoza
Apr 30, 2003·Arthritis Research & Therapy·Larry W Moreland
Sep 17, 2004·Biomaterials·I DonatiF Vittur
Sep 25, 2004·Journal of Cellular Biochemistry·Yi-Hsin HsuChien-Ho Chen
Dec 8, 2004·International Journal of Food Microbiology·Tsui-Chu YangChin-Fung Li
Feb 25, 2005·Osteoarthritis and Cartilage·V M Goldberg, J A Buckwalter
Mar 17, 2005·Biomaterials·Patrizia MarconSergio Paoletti
Oct 26, 2005·Osteoarthritis and Cartilage·K P H PritzkerW B van den Berg
May 23, 2006·Osteoarthritis and Cartilage·P G ConaghanR Altman
Sep 7, 2006·Endocrine-related Cancer·N Garcia de la TorreH E Turner
Sep 19, 2006·Histopathology·V KrennT Haupl
May 2, 2007·Osteoarthritis and Cartilage·S S GlassonE A Morris
Jun 5, 2007·Annals of the Rheumatic Diseases·C BoileauP Reboul
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·Eleonora MarsichSergio Paoletti
Dec 17, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yoshihisa MikiChiaki Hamanishi
Sep 3, 2010·Drugs & Aging·Roy D Altman
Dec 27, 2011·Bone·Jun PanLiyun Wang
Mar 20, 2012·Osteoarthritis and Cartilage·C KilkennyD G Altman
May 9, 2012·Arthritis Care & Research·Marc C HochbergUNKNOWN American College of Rheumatology
May 23, 2012·Advances in Orthopedics·Shachar PatchornikDror Robinson
May 18, 2013·Osteoarthritis and Cartilage·F OprenyeszkY Henrotin
Jun 19, 2013·Osteoarthritis and Cartilage·R F Loeser
Dec 3, 2013·Arthritis Research & Therapy·Stefan ToegelHans-Joachim Gabius
Mar 26, 2014·Nature Reviews. Rheumatology·Hang Fang, Frank Beier
May 27, 2014·Osteoarthritis and Cartilage·H IijimaH Kuroki
Jul 2, 2014·Histochemistry and Cell Biology·Stefan ToegelHans-Joachim Gabius
Feb 11, 2015·International Journal of Pharmaceutics·S KaderliO Jordan
Jan 23, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefan ToegelHans-Joachim Gabius
Feb 5, 2016·Nature Reviews. Rheumatology·Joana Osório
Nov 12, 2016·Osteoarthritis and Cartilage·F VeronesiM Fini
Dec 17, 2016·Scientific Reports·Daniela WeinmannHans-Joachim Gabius
Apr 5, 2017·Bio-medical Materials and Engineering·Fanny ComblainYves Henrotin
Nov 9, 2017·Joints·Ferdinando Priano
Dec 12, 2017·Osteoarthritis and Cartilage·X WangC Ding
Dec 23, 2017·BMC Musculoskeletal Disorders·Andrew ConcoffJeffrey Rosen
Jan 5, 2018·International Orthopaedics·Eric N BowmanFredrick M Azar
Citations
Feb 29, 2020·Cartilage·Elena TarriconePaola Brun
Mar 15, 2020·Journal of Biomedical Materials Research. Part a·Francesca ScognamiglioEleonora Marsich
Apr 17, 2020·Frontiers in Bioengineering and Biotechnology·Graziana MonacoMartin James Stoddart
May 30, 2020·Cells·Elena TarriconePaola Brun
Jul 28, 2020·Cells·G AbatangeloP Brun
Sep 11, 2020·BioMed Research International·J Raúl Olmos-ZuñigaMario Enrique Baltazares-Lipp
Jul 2, 2020·Cells·Matilde TschonMilena Fini
Jan 30, 2021·Tissue Engineering and Regenerative Medicine·Donggang MouXuming Yang
Feb 16, 2021·Cartilage·Stefania PaganiMilena Fini
Apr 4, 2021·Biomolecules·Riccardo LadièSabrina Bertini
Apr 27, 2021·Journal of Back and Musculoskeletal Rehabilitation·Betül BaşarHakan Başar
Sep 4, 2021·Cartilage·Akihiro NakahataHiroshi Kuroki
Sep 17, 2021·Cartilage·Ceyhun ÇağlarMahmut Uğurlu