PMID: 9426294Feb 28, 1998Paper

Effects of intracellular calcium on GABAA receptors in mouse cortical neurons

Pflügers Archiv : European journal of physiology
L G AguayoL S Satin

Abstract

Using the patch-clamp technique, we studied the effect of intracellular Ca2+ on Cl- current gated by type A gamma-aminobutyric acid receptors (GABAA) in mouse cortical neurons. When the rapid Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) was in the pipette solution, the GABA-activated Cl- current amplitude decreased over time to 49 +/- 7% of control. In contrast, equimolar replacement of BAPTA with ethylenebis(oxonitrilo)tetraacetate (EGTA) caused a 60 +/- 10% increase in GABA current. An increased intracellular Ca2+ concentration caused a transient augmentation of the GABA current. This effect of Ca2+ was concentration dependent (10 nM to 34 muM). Ca2+ increased the amplitude of the current by enhancing the maximal response to GABA rather than by changing the affinity of the receptor to GABA (EC50 = 5 +/- 0.4 muM vs. 7 +/- 0.3 muM). Both calmodulin (CaM) and a CaM kinase II inhibitor (200 muM) blocked the potentiating effect of Ca2+ suggesting that it was mediated by activation of CaM kinase II. We found that regulation of GABAA receptors by intracellular Ca2+ in cortical neurons has important physiological implications since the potentiating effect of increasing the intracellular Ca2+ on respo...Continue Reading

Citations

Jan 9, 2008·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Bernd Grünewald, Anna Wersing
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kurt C MarsdenReed C Carroll
Apr 13, 2011·The Journal of Biological Chemistry·Young-Hwan JoEric Boué-Grabot
Jul 16, 2004·Journal of Neurotrauma·Paulette B GoforthLeslie S Satin
Apr 30, 2004·Journal of Neurotrauma·Chang-Qing KaoLeslie S Satin
Nov 15, 2006·The European Journal of Neuroscience·C M Houston, T G Smart
Apr 20, 2007·The Journal of Biological Chemistry·Catriona M HoustonTrevor G Smart
Apr 1, 2009·The Journal of Physiology·Catriona M HoustonTrevor G Smart
Oct 21, 2011·Journal of Neurophysiology·Hyun-Jong JangDuck-Joo Rhie

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