Effects of intracellular magnesium on Kv1.5 and Kv2.1 potassium channels

European Biophysics Journal : EBJ
Paolo TammaroO Moran

Abstract

We characterized the effects of intracellular Mg(2+) (Mg(2+) (i)) on potassium currents mediated by the Kv1.5 and Kv2.1 channels expressed in Xenopus oocytes. Increase in Mg(2+) (i) caused a voltage-dependent block of the current amplitude, apparent acceleration of the current kinetics (explained by a corresponding shift in the steady-state activation) and leftward shifts in activation and inactivation dependencies for both channels. The voltage-dependent block was more potent for Kv2.1 [dissociation constant at 0 mV, K(d)(0), was approximately 70 mM and the electric distance of the Mg(2+) binding site, delta, was 0.2] than for the Kv1.5 channel [K(d)(0) approximately 40 mM and delta = 0.1]. Similar shifts in the voltage-dependent parameters for both channels were described by the Gouy-Chapman formalism with the negative charge density of 1 e(-)/100 A(2). Additionally, Mg(2+) (i) selectively reduced a non-inactivating current and increased the accumulation of inactivation of the Kv1.5, but not the Kv2.1 channel. A potential functional role of the differential effects of Mg(2+) (i) on the Kv channels is discussed.

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Citations

Jan 25, 2006·Experimental Brain Research·Loretta Ferrera, Oscar Moran
May 13, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amy L FirthSergey V Smirnov
Apr 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo H Pineda, Angeles B Ribera
Aug 12, 2006·European Biophysics Journal : EBJ·Thomas W ClaydonSteven J Kehl
Apr 25, 2013·The Journal of Physiology·Aiste AdomavicienePaolo Tammaro
Dec 18, 2009·British Journal of Pharmacology·R V FrolovS Singh
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Sep 26, 2013·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Hiroko YoshidaCharlotte Scheutz
Nov 13, 2015·British Journal of Pharmacology·Chau M TaPaolo Tammaro
Jun 16, 2017·British Journal of Pharmacology·Chau M TaPaolo Tammaro
Nov 25, 2006·Archives of Biochemistry and Biophysics·Kanigula MubagwaRegina Macianskiene

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