PMID: 8599248Dec 1, 1995Paper

Effects of intracellular pH on apoptosis in HL-60 human leukemia cells

Yonsei Medical Journal
H J Park

Abstract

The nature of the endonucleases responsible for DNA fragmentation in apoptosis has not yet been clearly defined. The intracellular acidity has been known to greatly affect apoptosis probably by affecting the activity of the endonucleases. In this study, the implication of pH in the apoptosis was investigated through the use of human HL-60 leukemia cells. The cells were incubated in media with different pH ranging from 3.5 to 7.5 for 4 hrs and the mode of cell death was investigated. The trypan blue exclusion assay showed that close to 25% and 90% of the cells were dead when incubated in pH 6.4 and pH 5.0 media, respectively. The agarose gel electrophoresis of DNA demonstrated that significant DNA fragmentation occurred in the HL-60 cells incubated in the pH 6.2-6.4 media for 4 hr indicating cell death by apoptosis. The electron microscopy study also demonstrated that many of the cells incubated in the pH 6.4 medium were in the process of apoptosis while the cells maintained in the pH 5.0 medium were dying by necrosis. The intracellular pH (pHi) of HL-60 cells was 6.6-6.9 when the extracellular pH (pHe) was 6.2-6.4. These results demonstrated that DNase I which has a maximal endonuclease activity near pH 7.0 may be responsible f...Continue Reading

Citations

Apr 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hai XiaoLeroy F Liu
Aug 29, 2013·Journal of Mathematical Biology·Maymona Al-HusariSteven D Webb
Jan 23, 1999·Bioelectromagnetics·Y YenC K Chou
Aug 26, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R HayashiY Otsuki

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