Effects of intracellular tyrosine residue mutation and carboxyl terminus truncation on signal transduction and internalization of the rat bradykinin B2 receptor.

The Journal of Biological Chemistry
Gregory N PradoPeter Polgar

Abstract

Presently, little is known of the amino acid motif(s) participating in bradykinin B2 receptor-mediated signal transduction processes. In this report we investigate the potential role of the two existing tyrosine (Tyr) residues in the intracellular regions and the carboxyl terminus in the regulatory function of this receptor. Rat-1 cells, which do not contain detectable bradykinin B2 receptor, were transfected with wild type and mutant receptor cDNAs. Tyr-131 and Tyr-321 were each mutated to corresponding alanine-, serine-, and phenylalanine-containing sequences. The last 34 amino acid residues of the carboxyl terminus were truncated. Rat-1 cells transfected with the mutant forms of the receptor cDNA including the truncated COOH-terminal cDNA all bound [3H]bradykinin with essentially the same Kd of approximately 2.2 nM as cells transfected with the wild type bradykinin B2 receptor. However, mutating Tyr-131 resulted in important changes in bradykinin-stimulated phosphoinositide turnover and arachidonate release. For example, exchanging Tyr-131 for alanine led to an 80% decreased arachidonate release (p < 0.005), 90% decrease in inositol phosphate (IP) accumulation (p < 0.001), with receptor uptake at 15 min remaining essentially...Continue Reading

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Citations

May 27, 1999·Electrophoresis·J Godovac-ZimmermannF Brianza
Feb 13, 1999·Progress in Neurobiology·M J Millan
Jun 14, 2002·Molecular and Cellular Endocrinology·Cecilia Castro-Fernández, P Michael Conn
Dec 20, 2002·International Immunopharmacology·Sylvia MuellerSiegmund Reissmann
Apr 1, 1999·Annual Review of Physiology·M BünemannM M Hosey
Mar 5, 2004·Chembiochem : a European Journal of Chemical Biology·Masaji IshiguroTakahiro Hirano
Feb 2, 1999·Clinical Reviews in Allergy & Immunology·F Marceau, D R Bachvarov
May 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhenhua HuangPeter Polgar
May 1, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sudakshina GhoshSerpil C Erzurum
Feb 29, 2000·The Journal of Biological Chemistry·S BastianJ L Paquet

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