Effects of intravitreal injection of KH902, a vascular endothelial growth factor receptor decoy, on the retinas of streptozotocin-induced diabetic rats

Diabetes, Obesity & Metabolism
J HuangY Luo

Abstract

KH902 is a fusion protein that can bind vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) through its binding ligand taken from the domains of VEGF receptor 1 and VEGF receptor 2 (VEGFR2). This study was to investigate the effects of intravitreal injection of KH902 on the retinas of streptozotocin-induced diabetic rats. Two weeks after induction of diabetes, the left eyes of diabetic rats in each group received an intravitreal injection of phosphate-buffered saline (PBS), Avastin or KH902 solution, respectively. Four weeks after intravitreal injection, retinal electrophysiological function and the integrity of inner blood retinal barrier (iBRB) were measured by electroretinogram and Evans blue perfusion. The protein levels of VEGF signal pathway were assayed by western blot. The expression and distribution of claudin-5 and occludin were analysed by double immunofluorescent staining under confocal microscope. The expression of VEGFR2 and PlGF was measured by immunohistochemistry. Four weeks after intravitreal injection, KH902-treated rats had better retinal electrophysiological function, less retinal vessel leakage and lower levels of VEGFR2, PI3K, AKT, p-AKT, p-ERK and p-SRC than PBS or Avastin-treate...Continue Reading

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Citations

Jun 7, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Fei WangXiaoxin Li
Mar 1, 2012·Journal of Ocular Biology, Diseases, and Informatics·Ghulam Mohammad, Mohammad Mairaj Siddiquei
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Apr 4, 2015·Current Eye Research·Xiaoyan JiangYan Luo
May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Qinghai PengWenhu Huang
Apr 17, 2021·Drug Discovery Today·Maryam Mahjoubin-TehranAmirhossein Sahebkar

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