Effects of ionic strength on the enzymatic hydrolysis of diluted and concentrated whey protein isolate

Journal of Agricultural and Food Chemistry
Claire I ButréHarry Gruppen

Abstract

To identify the parameters that affect enzymatic hydrolysis at high substrate concentrations, whey protein isolate (1-30% w/v) was hydrolyzed by Alcalase and Neutrase at constant enzyme-to-substrate ratio. No changes were observed in the solubility and the aggregation state of the proteins. With increasing concentration, both the hydrolysis rate and the final DH decreased, from 0.14 to 0.015 s(-1) and from 24 to 15%, respectively. The presence of 0.5 M NaCl decreased the rate of hydrolysis for low concentrations (to 0.018 s(-1) for 1% WPI), resulting in similar rates of hydrolysis for all substrate concentrations. The conductivity increase (by increasing the protein concentration, or by addition of NaCl) has significant effects on the hydrolysis kinetics, but the reason for this is not yet well understood. The results show the importance of conductivity as a factor that influences the kinetics of the hydrolysis, as well as the composition of the hydrolysates.

References

Sep 30, 2004·Journal of Dairy Science·H M FarrellH E Swaisgood
May 25, 2005·The Journal of Dairy Research·David SpellmanRichard J FitzGerald
Aug 5, 1994·Biotechnology and Bioengineering·P Gonzàlez-TelloE M Guadix
Oct 15, 2010·Journal of the Science of Food and Agriculture·Nathalie CreusotHarry Gruppen

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Citations

Jul 12, 2014·Analytical and Bioanalytical Chemistry·Claire I ButréPeter A Wierenga
Jan 30, 2014·Biotechnology and Bioengineering·N A HardtA J van der Goot
Mar 24, 2015·Biochimica Et Biophysica Acta·Claire I ButréPeter A Wierenga
Nov 6, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Roy J B M DelahaijePeter A Wierenga
Oct 26, 2016·Journal of Animal Science and Biotechnology·Sergio Salazar-VillaneaAntonius F B van der Poel
Jan 17, 2019·Annual Review of Food Science and Technology·Simon M Loveday
Apr 30, 2021·Biotechnology Reports·Leidy Maritza Sierra-Lopera, Jose Edgar Zapata-Montoya

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