Effects of ionic substitution on [Ca2+]i rises evoked by thrombin and PAF in human platelets

European Journal of Pharmacology
S O Sage, T J Rink

Abstract

We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF). Na+ substitution by choline did not affect the extent of the rise in [Ca2+]i evoked by either agonist. The response to thrombin, but not PAF, was slightly slowed. High K+ did not activate the cells, but the rises in [Ca2+]i evoked by both agonists were slowed and reduced. Shape change evoked by both agonists was little affected by either substitution. Aggregation evoked by PAF was reduced in high K+ but unaffected in choline. Thrombin-induced aggregation was unaffected by either substitution, even when the rise in [Ca2+]i was markedly reduced. The results suggest that the mechanism which generates Ca2+ fluxes in platelets is not voltage-dependent; but high K+ appears to interfere with the influx mechanism.

References

Feb 28, 1985·Biochemical and Biophysical Research Communications·V M Doyle, U T Rüegg
Apr 1, 1985·Cell Calcium·T J Rink, T Pozzan
Oct 29, 1974·Biochimica Et Biophysica Acta·M Steiner, T Tateishi
Dec 1, 1984·Journal of Cellular Physiology·E W GelfandS Grinstein
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T M Connolly, L E Limbird

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Citations

Feb 15, 1988·Experientia·T J Rink
Oct 15, 1989·Biochemical Pharmacology·C L KeralyJ Benveniste
Mar 22, 1988·European Journal of Pharmacology·T NakamuraC Yamato
Jan 1, 1989·Pharmacology & Therapeutics·J MorleyI Chapman
Jan 1, 1994·Pharmacology & Therapeutics·P Sargeant, S O Sage
Nov 1, 1988·Biological Reviews of the Cambridge Philosophical Society·J G Altin, F L Bygrave
Jun 30, 1988·Biochemical and Biophysical Research Communications·J G AltinF L Bygrave
Dec 18, 1989·FEBS Letters·W Siffert, J W Akkerman
Dec 1, 1991·Hypertension·T OshimaD A McCarron
Jul 1, 1995·Blood Pressure·I F IslimD G Beevers
Feb 1, 1990·The American Journal of Physiology·E L Stuenkel, S A Ernst
Oct 29, 1993·Biochimica Et Biophysica Acta·Y Tsunoda
Feb 22, 1988·Biochimica Et Biophysica Acta·S A Metz
Apr 1, 1988·Trends in Biochemical Sciences·W Siffert, J W Akkerman
Mar 27, 1989·Biochimica Et Biophysica Acta·J PalésA Gual

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