Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo

Journal of Applied Phycology
Aleksandra Inic-KanadaTalin Barisani-Asenbauer

Abstract

Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent...Continue Reading

References

May 29, 1992·Cell·J P Zhang, R S Stephens
Sep 1, 1987·Antimicrobial Agents and Chemotherapy·M E GonzálezL Carrasco
Jan 1, 1994·Methods in Enzymology·R G Rank, J A Whittum-Hudson
Mar 1, 1996·Infection and Immunity·B YasinE A Wagar
Sep 16, 1998·Antimicrobial Agents and Chemotherapy·G BergssonH Thormar
Mar 19, 1999·International Ophthalmology Clinics·T LietmanS Osaki
Feb 28, 2001·Sexually Transmitted Infections·C CogginsC Ellertson
May 22, 2001·Molecular Microbiology·R S StephensA Kubo
Dec 11, 2002·Best Practice & Research. Clinical Obstetrics & Gynaecology·Jane Norman
May 27, 2003·Investigative Ophthalmology & Visual Science·Ilene K GipsonCindy Leigh Russo
Sep 24, 2004·Current Medicinal Chemistry·Elsa B DamonteAlberto S Cerezo
Jul 15, 2006·PLoS Pathogens·Christopher B BuckJohn T Schiller
Dec 19, 2006·Journal of Medical Microbiology·Sanaa Fadel, Adrian Eley
Dec 18, 2007·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·N Haider, R Fisher
Sep 27, 2008·Virology Journal·Andreas GrassauerHermann Unger
Dec 23, 2008·The British Journal of Ophthalmology·S P MariottiJ Rose-Nussbaumer
Dec 24, 2010·PloS One·Andreas LeibbrandtAndreas Grassauer
Sep 20, 2011·Current Opinion in Biotechnology·Maria Daglia
Nov 9, 2011·Antimicrobial Agents and Chemotherapy·José A Fernández-RomeroThomas M Zydowsky
Aug 8, 2013·Investigative Ophthalmology & Visual Science·Elisabeth SteinTalin Barisani-Asenbauer
Jul 22, 2014·Lancet·Hugh R TaylorHeathcote Wright
Sep 16, 2015·Antimicrobial Agents and Chemotherapy·Keith LevendoskyJosé A Fernández-Romero
Oct 16, 2015·PloS One·Zhaochen LuoLing Zhao
Dec 24, 2015·Journal of Medical Virology·Laura B Talarico, Elsa B Damonte
Jul 18, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·James M McKimWilliam Blakemore
Nov 14, 2016·Journal of Photochemistry and Photobiology. B, Biology·Carolin RahnNicole Borel

❮ Previous
Next ❯

Citations

May 31, 2019·Marine Drugs·Maria Dolores TorresHerminia Domínguez
Jun 21, 2020·Drug Delivery and Translational Research·Susana RodriguesAna Grenha
Sep 12, 2020·International Journal of Environmental Research and Public Health·Sara García-PozaAna M M Gonçalves
Nov 27, 2020·Marine Drugs·Edisson-Mauricio Pacheco-QuitoMaría-Dolores Veiga
Jan 16, 2021·Carbohydrate Research·Edson Hipólito JúniorDiogo R B Ducatti
Dec 5, 2020·Vaccines·Marijana StojanovicAleksandra Inic-Kanada

❮ Previous
Next ❯

Software Mentioned

Tissue FaxSi
SPSS Statistics

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.