Effects of iron, copper, zinc, calcium, and magnesium on human lymphocytes in culture

Biological Trace Element Research
U CarpentieriM E Haggard

Abstract

The effects of simultaneous changes of calcium, magnesium, iron, copper, and zinc concentrations were evaluated in normal human T and B lymphocytes, cultured in cation-depleted media. Optimal concentrations for thymidine incorporation (TI) in both cell populations were Fe and Zn 15 microM and Cu 5 microM; for T cells Ca 2 mM and Mg 4 mM; for B cells Ca 4 mM and Mg 6 mM. TI decreased with increasing molarity of cations and the decrease was particularly apparent with Cu. Minimal amounts of Ca and Mg (0.5 mM) were necessary for growth, even in presence of optimal concentrations of Fe, Cu, and Zn. Fe and Cu showed synergistic stimulatory effects at low concentrations and synergistic inhibitory effects at high concentrations. Antagonism between Fe and Zn, Cu and Zn, and Ca and Zn was also demonstrated. CD4/CD8 increased with PHA stimulation in presence of Zn, and decreased with ConA stimulation in presence of Zn or Fe. The results demonstrate: (1) the relationship and interdependence of Fe, Cu, and Zn concentrations in modulating the growth of normal lymphocytes; (2) the stimulatory effects of Fe on B cells and Zn on CD8 positive cells; (3) the inhibitory effect of Cu at concentrations lower than those of Fe and Zn; (4) the requirem...Continue Reading

References

Dec 1, 1977·American Journal of Clinical Pathology·U CarpentieriL A Sordahl
Mar 1, 1975·Transplantation·J T LaneN R Ling
Jan 1, 1986·The British Journal of Nutrition·P S DowdP J Guillou
Apr 1, 1987·Journal of Biochemical and Biophysical Methods·U CarpentieriM E Haggard
Aug 1, 1985·Scandinavian Journal of Haematology·C W DaeschnerM E Haggard

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Citations

Jun 26, 2012·Biochemistry·Nema D JhurryPaul A Lindahl
Jul 1, 1995·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·M HockeH Bosseckert
Nov 1, 1996·Journal of the American Dietetic Association·K S Kubena, D N McMurray
Oct 19, 2017·Chemical Communications : Chem Comm·J H S K MonteiroA de Bettencourt-Dias
May 13, 2006·Journal of Materials Science. Materials in Medicine·K N LewisD A Puleo
May 28, 2019·Current Pharmaceutical Biotechnology·Ji-Hyun NamJeong-Ran Kim

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