Effects of iron overload on the bone marrow microenvironment in mice

PloS One
Yuchen ZhangAimin Meng

Abstract

Using a mouse model, Iron Overload (IO) induced bone marrow microenvironment injury was investigated, focusing on the involvement of reactive oxygen species (ROS). Mice were intraperitoneally injected with iron dextran (12.5, 25, or 50 mg) every three days for two, four, and six week durations. Deferasirox(DFX)125 mg/ml and N-acetyl-L-cysteine (NAC) 40 mM were co-administered. Then, bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated and assessed for proliferation and differentiation ability, as well as related gene changes. Immunohistochemical analysis assessed the expression of haematopoietic chemokines. Supporting functions of BM-MSCs were studied by co-culture system. In IO condition (25 mg/ml for 4 weeks), BM-MSCs exhibited proliferation deficiencies and unbalanced osteogenic/adipogenic differentiation. The IO BM-MSCs showed a longer double time (2.07±0.14 days) than control (1.03±0.07 days) (P<0.05). The immunohistochemical analysis demonstrated that chemokine stromal cell-derived factor-1, stem cell factor -1, and vascular endothelial growth factor-1 expression were decreased. The co-cultured system demonstrated that bone marrow mononuclear cells (BMMNCs) co-cultured with IO BM-MSCs had decreased colony fo...Continue Reading

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Feb 7, 2016·Biochimica Et Biophysica Acta·Adriana BorrielloFulvio Della Ragione
Mar 22, 2016·Annals of the New York Academy of Sciences·Emanuele Angelucci, Federica Pilo
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Feb 11, 2021·Clinical Kidney Journal·Takeshi Nakanishi, Takahiro Kuragano
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Jun 3, 2021·International Journal of Molecular Sciences·Verena PetzerDominik Wolf
Oct 14, 2021·PloS One·Supathra PhoaubonNarattaphol Charoenphandhu

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Methods Mentioned

BETA
chips
FACS
Flow Cytometry
biopsies
PCR

Software Mentioned

GraphPad
GraphPad Prism
Cell Quest

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