Effects of ischemic preconditioning in a pig model of large-for-size liver transplantation

Clinics
Antonio José Gonçalves LealUenis Tannuri

Abstract

In most cases of pediatric liver transplantation, the clinical scenario of large-for-size transplants can lead to hepatic dysfunction and a decreased blood supply to the liver graft. The objective of the present experimental investigation was to evaluate the effects of ischemic preconditioning on this clinical entity. Eighteen pigs were divided into three groups and underwent liver transplantation: a control group, in which the weights of the donors were similar to those of the recipients, a large-for-size group, and a large-for-size + ischemic preconditioning group. Blood samples were collected from the recipients to evaluate the pH and the sodium, potassium, aspartate aminotransferase and alanine aminotransferase levels. In addition, hepatic tissue was sampled from the recipients for histological evaluation, immunohistochemical analyses to detect hepatocyte apoptosis and proliferation and molecular analyses to evaluate the gene expression of Bax (pro-apoptotic), Bcl-XL (anti-apoptotic), c-Fos and c-Jun (immediate-early genes), ischemia-reperfusion-related inflammatory cytokines (IL-1, TNF-alpha and IL-6, which is also a stimulator of hepatocyte regeneration), intracellular adhesion molecule, endothelial nitric oxide synthase ...Continue Reading

Citations

Aug 5, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Liu HuiYang Shu-Long
Mar 19, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Arumugam Suyavaran, Chinnasamy Thirunavukkarasu
Apr 25, 2017·Pediatric Transplantation·Ana Cristina Aoun TannuriUenis Tannuri
Aug 14, 2015·Acta Cirúrgica Brasileira·Marco Antonio Correa Guimarães FilhoMarcos Bettini Pitombo
Oct 7, 2015·Frontiers in Genetics·Karina GutierrezVilceu Bordignon
Jun 29, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Alessandro Rodrigo BelonUenis Tannuri

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Methods Mentioned

BETA
biopsy
light microscopy
electrophoresis
PCR

Software Mentioned

R
Excel

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