Abstract
To study the effects of sense and antisense KAI1 genes on the growth and invasion of human hepatocellular carcinoma (HCC) cell line MHCC97-H. KAI1 sense and antisense eukaryotic expression plasmids were constructed using subclone technique and transfected into MHCC97-H cells respectively by DOTAP liposome. After successful transfection was confirmed, in vitro growth curve, cell cycles, plate clone formation efficiency, invasive ability in Boyden Chamber assay and ultrastructural morphology were studied. KAI1 sense and antisense genes had no significant effects on the cell growth curve and cell cycles. After transfection with sense KAI1 gene, decreased invasive ability in Boyden Chamber assay and decreased amount of mitochondria, but no significant changes of plate clone formation efficiency were observed in MHCC97-H-S cells. The plate clone formation efficiency and invasive ability in Boyden Chamber assay were significantly increased in MHCC97-H-AS cells, after transfection with antisense KAI1 gene. Furthermore, increased amount of mitochondria, rough endoplasmic reticulum, Golgi apparatus and expanded endoplasmic reticulum were also noted in MHCC97-H-AS cells. Changes of KAI1 expression in HCC cells may alter their invasive an...Continue Reading
References
May 12, 1995·Science·J T DongJ C Barrett
Apr 1, 1997·Genomics·J T DongJ T Isaacs
Apr 3, 1998·Oncogene·A TakaokaK Imai
Apr 29, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M AdachiM Miyake
Jul 22, 1998·Oncogene·A WhiteJ C Barrett
Sep 15, 1998·The American Journal of Pathology·C I HuangM Miyake
Dec 22, 1999·European Journal of Immunology·N ShibagakiH Hamada
Nov 7, 2001·The American Journal of Pathology·F S LiuC H Lu
Jan 31, 2002·World Journal of Gastroenterology : WJG·Y LiW H Bao
Apr 2, 2002·World Journal of Gastroenterology : WJG·Lun-Xiu Qin, Zhao-You Tang
May 29, 2002·Pathology International·Kazuki NabeshimaTetsuro Sameshima
Jun 25, 2002·Gynecologic Oncology·Christopher D HouleBarbara J Davis
Jun 27, 2002·Seminars in Cancer Biology·Manuel PatarroyoIsmo Virtanen
Jul 10, 2002·Acta Oncologica·Alan WellsDouglas A Lauffenburger
Aug 9, 2002·Oral Oncology·Y ImaiT Fujibayashi
Dec 24, 2002·Prostate Cancer and Prostatic Diseases·K TozawaK Kohri
Feb 20, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mario WernickeAna Alcestes Morandi
Feb 22, 2003·Biochimie·Paola PoggialiCarmen Cimmino
Feb 27, 2003·Journal of Gastroenterology and Hepatology·Jian-Min YangWei-Wen Liu
Mar 19, 2003·Experimental & Molecular Medicine·Bokeun JeeHansoo Lee
Apr 15, 2003·The Journal of Pathology·Hye Seung LeeWoo Ho Kim
Apr 30, 2003·The Journal of Biological Chemistry·Piia VehviläinenJorma Keski-Oja
May 16, 2003·Pathology, Research and Practice·Yasuhiro ItoAkira Miyauchi
May 27, 2003·International Journal of Cancer. Journal International Du Cancer·Béatrice Nawrocki-RabyPhilippe Birembaut
Jun 26, 2003·Cancer Science·Hitoshi KurokawaMasaki Yanagishita
Citations
Dec 2, 2014·Cellular Signalling·Sina UpheberUte Reuning
Apr 18, 2009·Experimental Cell Research·Zlatna RusevaUte Reuning
Sep 30, 2008·Cellular Signalling·C K Miranti
Nov 22, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Jian-Min YangZai-Yuan Ye
Nov 20, 2012·Oncogene·Y SuhS-J Lee
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hyun-Ah LeeHansoo Lee