PMID: 9422575Jan 9, 1998Paper

Effects of keratinocyte growth factor on the proliferation and radiation survival of human squamous cell carcinoma cell lines in vitro and in vivo

International Journal of Radiation Oncology, Biology, Physics
S NingS J Knox

Abstract

Keratinocyte growth factor (KGF) has potent mitogenic activity on normal epithelial cells and has been found to enhance intestinal crypt cell survival in irradiated mice and to prevent radiation and chemotherapy-induced mucositis in animal models. The purpose of the study reported here is to investigate the effect of recombinant human KGF on the proliferation and survival of human squamous carcinoma cell lines following irradiation. The level of KGF receptor (KGFR) mRNA in normal Balb/Mk cell line and human head and neck squamous carcinoma cell lines was assessed using a RNase protection assay. The clonogenic assay and MTT assay were used to study the proliferative effects of KGF on human tumor cell lines and Balb/MK cell line in vitro. Effects of KGF on in vivo tumor growth and radiosensitivity were studied in three KGFR-positive human squamous cell carcinoma xenografts (FaDu, Detroit 562 and A431) in nude mice, and a murine KGFR-negative melanoma tumor (B16) in Balb/c mice. Seven of 10 tumor cell lines studied expressed KGFR mRNA. None of these tumor cell lines showed enhanced proliferation when exposed to KGF for 2 days or less. Prolonged exposure to KGF for 7 days or longer resulted in low level stimulation of proliferation...Continue Reading

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Citations

Jun 7, 2005·International Journal of Radiation Oncology, Biology, Physics·Wolfgang DörrKathrin Spekl
Aug 17, 2002·International Journal of Radiation Oncology, Biology, Physics·Wolfgang DörrCatherine L Farrell
Jan 3, 2003·Oral Oncology·Andrei Barasch, Douglas E Peterson
Feb 19, 2002·British Journal of Haematology·Karin Wirth, Roland Mertelsmann
Jun 22, 2006·Journal of the National Cancer Institute·Paul W Finch, Jeffrey S Rubin
Apr 11, 2014·International Journal of Molecular Medicine·Y M LuoY F Zhou
Jan 2, 2010·Cancer Treatment Reviews·Piotr Winczura, Jacek Jassem
Dec 17, 2003·The Journal of Investigative Dermatology·Daniela Elena CosteaAnne Christine Johannessen
Aug 21, 2007·Experimental and Molecular Pathology·Mitsuhiro KudoZenya Naito
May 10, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Wolfgang DörrKathrin Spekl
Mar 23, 2011·Expert Opinion on Drug Metabolism & Toxicology·Christian WeigeltGuido Kobbe
May 22, 2009·Expert Opinion on Biological Therapy·Uzma Athar, Teresa C Gentile
Jun 19, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mitchell MachtayK Kian Ang
May 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David M BrizelWilliam Mendenhall
Nov 22, 2005·International Journal of Radiation Biology·Wolfgang DörrKathrin Spekl
Oct 5, 2001·American Journal of Clinical Oncology·P OkunieffI Ding
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wei WangLurong Zhang

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